c-MET Protein, Human, Recombinant (ECD, His & hFc Tag)

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c-MET Protein, Human, Recombinant (ECD, His & hFc Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml
蛋白构建
A DNA sequence encoding the extracellular domain (Met1-Thr932) of human c-Met (NP_000236.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Glu 25
分子量
The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 57.8 kDa and 130 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液
Lyophilized from sterile PBS, 8% Trehalose, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

c-MET Protein, Human, Recombinant (ECD, His & hFc Tag): 图片

Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100μl/well) can bind Human c-MET / HGFR with a linear range of 0.31-160 ng/ml.

c-MET Protein, Human, Recombinant (ECD, His & hFc Tag): 别称

AUTS9 Protein, Human; c-Met Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human

c-MET 背景信息

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
全称
MET proto-oncogene, receptor tyrosine kinase
参考文献
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
  • High-Affinity Human Anti-c-Met IgG Conjugated to Oxaliplatin as Targeted Chemotherapy for Hepatocellular Carcinoma
    Author
    Ma, Y;Zhang, M;Wang, J;Huang, X;Kuai, X;Zhu, X;Chen, Y;Jia, L;Feng, Z;Tang, Q;Liu, Z;
    Year
    2019
    Journal
    Front Oncol
    Application
    ELISA
  • cIRCR201-dPBD, a Novel Pyrrolobenzodiazepine Dimer-Containing Site-Specific Antibody–Drug Conjugate Targeting c-Met Overexpression Tumors
    Author
    Min, B;Jin, J;Kim, H;Her, N;Park, C;Kim, D;Yang, J;Hwang, J;Kim, E;Choi, M;Song, H;Nam, D;Yoon, Y;
    Year
    2020
    Journal
    ACS Omega
    Application
    ELISA
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