c-MET Protein, Human, Recombinant (ECD, His Tag)

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c-MET Protein, Human, Recombinant (ECD, His Tag): 产品信息

纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to bind human HGF (Cat: 10463-HNAS) in functional ELISA.
蛋白构建
A DNA sequence encoding the extracellular domain of human c-Met (NP_000236.2) (Met1-Thr932) was fused with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Glu 25
分子量
The mature form of recombinant human c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 919 amino acids and has a predicted molecular mass of 103 (α=33 +β=70) kDa. As a result of glycosylation, rh c-MET heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 85 kDa respectively in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

c-MET Protein, Human, Recombinant (ECD, His Tag): 图片

Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 10 μg/ml (100 μl/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 5.28 μg/ml.

c-MET Protein, Human, Recombinant (ECD, His Tag): 别称

AUTS9 Protein, Human; c-Met Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human

c-MET 背景信息

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
全称
MET proto-oncogene, receptor tyrosine kinase
参考文献
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
  • Anti-MET immunoPET for non-small cell lung cancer using fully human antibody fragments
    Author
    Li, K;Tavare, R;Zettlitz, KA;Mumenthaler, SM;Mallick, P;Zhou, Y;Marks, JD;Wu, AM;
    Year
    2014
    Journal
    Mol. Cancer Ther.
    Application
    Phage library selection
  • Detection of colonic neoplasia in vivo using near-infrared-labeled peptide targeting cMet
    Author
    Wu, X;Zhou, J;Wang, F;Meng, X;Chen, J;Chang, TS;Lee, M;Li, G;Li, X;Appelman, HD;Kuick, R;Wang, TD;
    Year
    2019
    Journal
    Sci Rep
    Application
    biopanning
  • Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors
    Author
    Yuan, X;Sun, Z;Yuan, Q;Hou, W;Liang, Q;Wang, Y;Mo, W;Wang, H;Yu, M;
    Year
    2020
    Journal
    Invest New Drugs
    Application
    cell-based
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