EpCAM Protein, Human, Recombinant (ECD, hFc Tag)
EpCAM Protein, Human, Recombinant (ECD, hFc Tag): 产品信息
纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the extracellular domain (Met1-Lys265) of human EpCAM (NP_002345.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Gln 24
分子量
The recombinant human EpCAM/Fc chimera is a disulfide-linked homodimeric protein after removal of the signal peptide. The monomer consists of 480 amino acids and has a calculated molecular mass of 54 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EpCAM/Fc is approximately 65-70 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EpCAM Protein, Human, Recombinant (ECD, hFc Tag): 图片
EpCAM Protein, Human, Recombinant (ECD, hFc Tag): 别称
DIAR5 Protein, Human; EGP-2 Protein, Human; EGP314 Protein, Human; EGP40 Protein, Human; ESA Protein, Human; HNPCC8 Protein, Human; KS1/4 Protein, Human; KSA Protein, Human; M4S1 Protein, Human; MIC18 Protein, Human; MK-1 Protein, Human; TACSTD1 Protein, Human; TROP-1 Protein, Human; TROP1 Protein, Human
EpCAM 背景信息
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
Human EpCAM 蛋白
Mouse EpCAM 蛋白
Cynomolgus EpCAM 蛋白
Cynomolgus, Rhesus EpCAM 蛋白
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A fluorescence turn-on biosensor based on graphene quantum dots (GQDs) and molybdenum disulfide (MoS2) nanosheets for epithelial cell adhesion molecule (EpCAM) detectionAuthorShi, J;Lyu, J;Tian, F;Yang, M;Year2016JournalBiosens BioelectronApplicationcontrol
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