FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag)
Cat: CT009-H08H
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): 产品信息
纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its ability to bind human IgG1 (Cat: 10702-HNAC) in a functional ELISA.
2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) bound to Conbercept was 0.1 μM.
2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) bound to Conbercept was 0.1 μM.
蛋白构建
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61770-1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Ala 24 & Ile 21
分子量
The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): 图片
Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio;
Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated (Cat. CT009-H08H-B) bound to Conbercept was 0.1 μM.
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): 别称
alpha-chain Protein, Human; FCRN Protein, Human
FCGRT & B2M Heterodimer 背景信息
Cynomolgus FCGRT & B2M Heterodimer 蛋白
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The neonatal Fc receptor is a pan-echovirus receptorAuthorMorosky, S;Evans, A;Lemon, K;Schmus, S;Bakkenist, C;Coyne, C;Year2018JournalbioRxivApplicationpull down
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Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoformsAuthorWada, R;Matsui, M;Kawasaki, N;Year2018JournalMAbsApplicationSPR
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