IL-33 Protein, Human, Recombinant
IL-33 Protein, Human, Recombinant: 产品信息
纯度
> 98 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
蛋白构建
A DNA sequence encoding the C-terminal segment of human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
NP No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-33 Protein, Human, Recombinant: 图片
Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
IL-33 Protein, Human, Recombinant: 别称
C9orf26 Protein, Human; DVS27 Protein, Human; IL1F11 Protein, Human; NF-HEV Protein, Human; NFEHEV Protein, Human
IL-33 背景信息
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
全称
interleukin 33
Related Pathways
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IL1 signaling pathway
参考文献
- Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
- Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
Cynomolgus IL-33 蛋白
Canine IL-33 蛋白
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Altered serum levels of IL-33 in patients with advanced systolic chronic heart failure: correlation with oxidative stressAuthorZhang, HF;Xie, SL;Chen, YX;Mai, JT;Wang, JF;Zhu, WL;Zhu, LG;Year2012JournalJ Transl MedApplicationcell-based (culture)
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IL-33 signaling fuels outgrowth and metastasis of human lung cancerAuthorWang, C;Chen, Z;Bu, X;Han, Y;Shan, S;Ren, T;Song, W;Year2016JournalBiochem. Biophys. Res. Commun.Applicationcell-based
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Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in PsoriasisAuthorLei, H;Li, X;Jing, B;Xu, H;Wu, Y;Year2017JournalPLoS ONEApplicationcell-based
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