LYPLA2 Protein, Human, Recombinant (His Tag)

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LYPLA2 Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 96 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the human LYPLA2 (O95372) (Met 1-Val 231) was expressed, with a polyhistide tag at the N-terminus.
Uniprot No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant human LYPLA2 consisting of 242 amino acids and has a calculated molecular mass of 26.2 kDa as migrated in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 50mM Tris, 0.05% Bring, pH 8.2
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LYPLA2 Protein, Human, Recombinant (His Tag): 图片

LYPLA2 Protein, Human, Recombinant (His Tag): 别称

APT-2 Protein, Human; APT2 Protein, Human; DJ886K2.4 Protein, Human

LYPLA2 背景信息

Lysophospholipase II (LYPLA2, LPL-II, or LysoPLA II), also known as Acyl-protein thioesterase 2 (APT-2), belongs to the AB hydrolase 2 family. This enzyme has lysophospholipase activity and may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acyl-protein thioesterase 1 (APT-1) and Acyl-protein thioesterase 2 (APT-2) are cytosolic lysophospholipids hydrolyzing enzymes. The serum activity of APT-1 may play an important role in the determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. APT-2/LYPLA2 is expressed both in CHO-K1 and HeLa cells and its overexpression increased the deacylation rate of single acylated GAP-43 and affected the steady-state localization of diacylated GAP-43 and H-Ras. Thus, the results demonstrate that APT-2/LYPLA2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution.
全称
lysophospholipase II
参考文献
  • Satou M, et al. (2010) Identification and characterization of acyl-protein thioesterase 1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium. Endocrinology. 151(10): 4765-75.
  • Tomatis VM, et al. (2010) Acyl-protein thioesterase 2 catalyzes the deacylation of peripheral membrane-associated GAP-43. PLoS One. 5(11): e15045.
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