CD86 Protein, Mouse, Recombinant (His Tag)

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CD86 Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 92 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its binding ability in a functional ELISA . Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear range of 6.4-800 ng/ml.
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Mouse
预测 N 端
Val 24
分子量
The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD86 Protein, Mouse, Recombinant (His Tag): 图片

Measured by its binding ability in a functional ELISA. Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-800 ng/ml.

CD86 Protein, Mouse, Recombinant (His Tag): 别称

B7 Protein, Mouse; B7-2 Protein, Mouse; B7.2 Protein, Mouse; B70 Protein, Mouse; Cd28l2 Protein, Mouse; CLS1 Protein, Mouse; ETC-1 Protein, Mouse; Ly-58 Protein, Mouse; Ly58 Protein, Mouse; MB7 Protein, Mouse; MB7-2 Protein, Mouse; TS/A-2 Protein, Mouse

CD86 背景信息

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B7), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
全称
CD86 antigen
研究领域
参考文献
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
  • PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
    Author
    Zhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;
    Year
    2019
    Journal
    Immunity
  • A functional antibody cross-reactive to both human and murine cytotoxic T-lymphocyte-associated protein 4 via binding to an N-glycosylation epitope
    Author
    Li, D;Li, J;Chu, H;Wang, Z;
    Year
    2020
    Journal
    MAbs
  • PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2
    Author
    Xu, X;Hou, B;Fulzele, A;Masubuchi, T;Zhao, Y;Wu, Z;Hu, Y;Jiang, Y;Ma, Y;Wang, H;Bennett, EJ;Fu, G;Hui, E;
    Year
    2020
    Journal
    J. Cell Biol.
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