SARS-CoV-2 (2019-nCoV) Nucleocapsid (T205I) Protein (His Tag)

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SARS-CoV-2 (2019-nCoV) Nucleocapsid (T205I) Protein (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE.
内毒素
Please contact us for more information.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid (YP_009724397.2, with mutation T205I) (Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus. The mutation were identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.
表达宿主
E. coli
种属
SARS-CoV-2
预测 N 端
Met
分子量
The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid consists of 426 amino acids and predicts a molecular mass of 46.6 kDa.
缓冲液
Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SARS-CoV-2 (2019-nCoV) Nucleocapsid (T205I) Protein (His Tag): 图片

Coronavirus Nucleocapsid 背景信息

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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