人冠状病毒核衣壳(Nucleocapsid)蛋白

核衣壳蛋白(N 蛋白)

核衣壳蛋白(N蛋白)是冠状病毒中含量最丰富的蛋白。 N蛋白是一种具有高度免疫原性的磷蛋白,基因序列非常保守。冠状病毒的N蛋白通常被用作诊断检测标志物。在病毒体组装过程中,N蛋白与病毒RNA结合并形成螺旋核衣壳。高含量的N蛋白以及N蛋白的高亲水性被认为有利于冠状病毒感染细胞后产生有效免疫力。

核衣壳蛋白(N 蛋白)功能

N蛋白是唯一存在于核衣壳中的蛋白。它由两个独立的结构域组成,一个N末端结构域(NTD)和一个C末端结构域(CTD)。两者都能够在体外结合RNA,但结合的机制不一样。

N蛋白被大量磷酸化。有研究表明,磷酸化会触发N蛋白结构变化,从而增强对非病毒RNA的亲和力。N蛋白以串珠型构象结合病毒基因组。已鉴定出N蛋白的两种特异性RNA底物,TRS和基因组包装信号。

N蛋白还可以和M蛋白以及复制酶复合体的关键组分nsp3结合。这些蛋白质相互作用可能有助于将病毒基因组结合在复制酶-转录酶复合物(RTC)上,进而将衣壳化的基因组包装成病毒颗粒。
(Ref: Anthony R. Fehr and Stanley Perlman.Coronaviruses: An Overview of Their Replication and Pathogenesis.Methods Mol Biol. 2015)

 

核衣壳蛋白(N蛋白)相关试剂

重组蛋白

SARS-CoV-2(2019-nCoV)
SARS-CoV
MERS-CoV
HCoV-229E
HCoV-NL63
HCoV-OC43
HCoV-HKU1
高纯度;昆虫细胞表达, 更接近天然结构
Cat#:40588-V08B
Purity: >95%
CoV: SARS-CoV-2 (2019-nCoV)

抗体

SARS-CoV-2(2019-nCoV)
SARS-CoV
MERS-CoV
高灵敏度
Cat#:40143-R019
Detection Limit: 0.5ng
CoV: SARS-CoV-2 (2019-nCoV)

Sample: 2019-nCoV NP Protein
(Cat# 40588-V08B)
Lane A: 30ng
Lane B: 10ng
Lane C: 5ng
Lane D: 2ng
Lane E: 1ng
Lane F: 0.5ng

N 蛋白检测试剂盒

Cat#:KIT40588
Assay range: 93.75-6000 pg/mL
Specificity:
Verified by high purity SARS-CoV-2 (2019-nCoV) Nucleocapsid / N Protein (Cat# 40588-V08B) recombinant protein.
Cat#   Antigen More
40068-V08B   MERS-CoV Nucleocapsid Protein

Expressed Host Insect Cells
Tag His
40143-V08B   SARS-CoV Nucleocapsid Protein

Expressed Host Insect Cells
Tag His
40588-V07E   SARS-CoV-2 Nucleocapsid Protein

Expressed Host E. coli
Tag His
40588-V08B   SARS-CoV-2 Nucleocapsid Protein

Expressed Host Insect Cells
Tag His
40588-V08B-B   SARS-CoV-2 Nucleocapsid Protein(Biotinylated)

Expressed Host Insect Cells
Tag His
40588-V27B-B   SARS-CoV-2 Nucleocapsid Protein(Biotinylated)

Expressed Host Insect Cells
Tag AVI-His
40640-V07E   HCoV-229E Nucleocapsid Protein

Expressed Host E. coli
Tag His
40641-V07E   HCoV-NL63 Nucleocapsid Protein

Expressed Host E. coli
Tag His
40642-V07E   HCoV-HKU1 Nucleocapsid Protein

Expressed Host E. coli
Tag His
40643-V07E   HCoV-OC43 Nucleocapsid Protein

Expressed Host E. coli
Tag His
Cat#   Antigen More
40588-R0004   SARS-CoV-2 Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40588-T62   SARS-CoV-2 Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40143-R019   SARS-CoV-2 Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40143-MM05   SARS-CoV Nucleocapsid Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40143-MM08   SARS-CoV Nucleocapsid Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40143-R001   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40143-R004   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40143-R040   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40143-R040-H   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40143-RP01   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40143-T62   SARS-CoV Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
100213-RP02   MERS-CoV Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40068-MM10   MERS-CoV Nucleocapsid Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40068-RP01   MERS-CoV Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40068-RP02   MERS-CoV Nucleocapsid Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
Cat#: KIT40588
Assay range: 93.75-6000 pg/mL
Specificity: The kit has been verified by high purity SARS-CoV-2 (2019-nCoV) Nucleocapsid / N Protein (Cat# 40588-V08B) recombinant protein. It can recognize recombinant SARS-CoV Nucleocapsid / N Protein (His Tag) (Cat# 40143-V08B), but no react with recombinant MERS-CoV Nucleoprotein / NP protein (His Tag) (Cat# 40068-V08B).
Recombinant Antibody Production