人冠状病毒Spike抗原

棘突蛋白Spike

冠状病毒棘突蛋白是I类病毒融合蛋白,可介导病毒与宿主受体的结合。 N-糖基化的S蛋白在不同冠状病毒之间的长度差异很大,大约1100aa-1600aa,分子量最高可达220kDa。三聚体Spike蛋白形成18-23nm长的棍状尖峰,分布在冠状病毒膜表面。Spike蛋白是宿主中和抗体的重要作用位点以及疫苗设计的关键靶点。

Spike蛋白含有2个不同功能的亚基:S1亚基通常呈球形,含有受体结合结构域,可与宿主受体结合;S2亚基促进病毒与宿主细胞的膜融合过程。

β冠状病毒Spike蛋白的S1亚基含有多个结构域A-D,其中结构域A和B组成受体结构域RBD。S2亚基是高度保守的,其中含有促进病毒与细胞融合的关键蛋白片段,包括融合肽,两个七肽重复区(HR1和HR2)以及跨膜结构域。S2的跨膜结构域在不同属的冠状病毒间也是非常保守的。
(Ref: R.J.G. Hulswit.Coronavirus Spike Protein and Tropism Changes.Advances in Virus Research. 2016)

Spike与受体相互作用

Spike蛋白结合宿主受体是病毒感染细胞的关键步骤。不同的人类冠状病毒会利用不同的细胞表面受体进行结合。

Genera HCoV Types Host receptors
Alphacoronavirus HCoV-229E APN ( aminopeptidase N, CD13 )
Alphacoronavirus HCoV-NL63 ACE2 ( angiotensin-converting enzyme 2 )
Betacoronavirus HCoV-HKU1 O-ac Sia
Betacoronavirus HCoV-OC43 O-ac Sia
Betacoronavirus MERS-CoV DPP4 ( dipeptidyl peptidase 4 )
Betacoronavirus SARS-CoV ACE2 ( angiotensin-converting enzyme 2 )
Betacoronavirus SARS-CoV-2 (2019-nCoV) ACE2 ( angiotensin-converting enzyme 2 )
 

Spike蛋白相关试剂

重组蛋白

高纯度;真核细胞表达,更接近天然结构;与受体分子的结合活性
纯度:>95% by SDS-PAGE & SEC-HPLC
 
活性:结合ACE2
Cat#:40592-V05H
Purity: >95%
Antigen: Spike S1-RBD
CoV: SARS-CoV-2 (2019-nCoV)
Cat#   Description More
40601-V08H   HCoV-229E Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
40605-V08B   HCoV-229E Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40021-V08H   HCoV-HKU1 Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
40602-V08H   HCoV-HKU1 Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
40606-V08B   HCoV-HKU1 Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40600-V08H   HCoV-NL63 Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
40604-V08B   HCoV-NL63 Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40607-V08H1   HCoV-OC43 Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
40607-V08B   HCoV-OC43 Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40071-V08B1   MERS-CoV Spike RBD Protein

Expressed Host Insect Cells
Tag His
40071-V31B1   MERS-CoV Spike RBD Protein

Expressed Host Insect Cells
Tag rFc
40069-V08B1   MERS-CoV Spike S1 Protein

Expressed Host Insect Cells
Tag His
40069-V08H   MERS-CoV Spike S1 Protein

Expressed Host HEK293 Cells
Tag His
HPLC-40069-V08H   MERS-CoV Spike S1 Protein (HPLC-verified)

Expressed Host HEK293 Cells
Tag His
40069-V08B   MERS-CoV Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40070-V08B   MERS-CoV Spike S2 Protein

Expressed Host Insect Cells
Tag His
40150-V05H   SARS-CoV Spike RBD Protein

Expressed Host HEK293 Cells
Tag mFc
40150-V08B2   SARS-CoV Spike RBD Protein

Expressed Host Insect Cells
Tag His
40150-V31B2   SARS-CoV Spike RBD Protein

Expressed Host Insect Cells
Tag rFc
40150-V05H1   SARS-CoV Spike S1 Protein

Expressed Host HEK293 Cells
Tag mFc
40150-V08B1   SARS-CoV Spike S1 Protein

Expressed Host Insect Cells
Tag His
40150-V08B1-B   SARS-CoV Spike S1 Protein

Expressed Host Insect Cells
Tag His
40634-V08B   SARS-CoV Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40150-V08B3   SARS-CoV Spike S2 Protein

Expressed Host Insect Cells
Tag His
40592-V08H4   SARS-CoV-2 Spike RBD (A435S) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H6   SARS-CoV-2 Spike RBD (F342L) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H8   SARS-CoV-2 Spike RBD (G476S) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H7   SARS-CoV-2 Spike RBD (K458R) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H2   SARS-CoV-2 Spike RBD (N354D) Protein

Expressed Host HEK293 Cells
Tag His
40592-V05H1   SARS-CoV-2 Spike RBD (V367F) Protein

Expressed Host HEK293 Cells
Tag mFc
40592-V08H1   SARS-CoV-2 Spike RBD (V367F) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H5   SARS-CoV-2 Spike RBD (V483A) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08H9   SARS-CoV-2 Spike RBD (W436R) Protein

Expressed Host HEK293 Cells
Tag His
40592-V08B   SARS-CoV-2 Spike RBD Protein

Expressed Host Insect Cells
Tag His
40592-V08H   SARS-CoV-2 Spike RBD Protein

Expressed Host HEK293 Cells
Tag His
40592-V31H   SARS-CoV-2 Spike RBD Protein

Expressed Host HEK293 Cells
Tag rFc
40592-VNAH   SARS-CoV-2 Spike RBD Protein

Expressed Host HEK293 Cells
Tag
40592-V08B-B   SARS-CoV-2 Spike RBD Protein (Biotinylated)

Expressed Host Insect Cells
Tag His
40592-V08H-B   SARS-CoV-2 Spike RBD Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag His
40592-V27B-B   SARS-CoV-2 Spike RBD Protein (Biotinylated)

Expressed Host Insect Cells
Tag AVI & His
40592-V27H-B   SARS-CoV-2 Spike RBD Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag AVI & His
40592-V02H   SARS-CoV-2 Spike RBD Protein (HPLC-verified)

Expressed Host HEK293 Cells
Tag Fc
40592-V05H   SARS-CoV-2 Spike RBD Protein (HPLC-verified)

Expressed Host HEK293 Cells
Tag mFc
40591-V08H3   SARS-CoV-2 Spike S1 (D614G) Protein (HPLC-verified)

Expressed Host HEK293 Cells
Tag His
40591-V02H   SARS-CoV-2 Spike S1 Protein

Expressed Host HEK293 Cells
Tag Fc
40591-V05H1   SARS-CoV-2 Spike S1 Protein

Expressed Host HEK293 Cells
Tag mFc
40591-V08B1   SARS-CoV-2 Spike S1 Protein

Expressed Host Insect Cells
Tag His
40591-V08H-B   SARS-CoV-2 Spike S1 Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag His
40591-V27H-B   SARS-CoV-2 Spike S1 Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag AVI & His
40591-V08H   SARS-CoV-2 Spike S1 Protein (HPLC-verified)

Expressed Host HEK293 Cells
Tag His
40589-V08B1   SARS-CoV-2 Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag His
40589-V27B-B   SARS-CoV-2 Spike S1+S2 ECD Protein

Expressed Host Insect Cells
Tag AVI & His
40591-V41H   SARS-CoV-2 Spike S1-NTD Protein

Expressed Host HEK293 Cells
Tag Fc & AVI
40591-V41H-B   SARS-CoV-2 Spike S1-NTD Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag Fc & AVI
40591-V49H-B   SARS-CoV-2 Spike S1-NTD Protein (Biotinylated)

Expressed Host HEK293 Cells
Tag His & AVI
40590-V02H   SARS-CoV-2 Spike S2 Protein

Expressed Host HEK293 Cells
Tag Fc
40590-V05B   SARS-CoV-2 Spike S2 Protein

Expressed Host Insect Cells
Tag mFc
40590-V08B   SARS-CoV-2 Spike S2 Protein

Expressed Host Insect Cells
Tag C-His
Cat#   Description More
40021-MM07   HCoV-HKU1 Spike Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40021-R028   HCoV-HKU1 Spike Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40021-RP01   HCoV-HKU1 Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40021-T60   HCoV-HKU1 Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-R723   MERS-CoV Spike Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40069-RP01   MERS-CoV Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-T30   MERS-CoV Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-T44   MERS-CoV Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-T62   MERS-CoV Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40070-MM02   MERS-CoV Spike Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
100207-RP02   MERS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
100208-RP02   MERS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
100209-RP02   MERS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-MM23   MERS-CoV Spike S1 Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40069-T48   MERS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40069-T52   MERS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
100210-RP02   MERS-CoV Spike S2 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40070-MM11   MERS-CoV Spike S2 Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40070-T60   MERS-CoV Spike S2 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40150-D001   SARS-CoV Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-T62-COV2   SARS-CoV Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40150-MM02   SARS-CoV Spike S1 Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40150-MM08   SARS-CoV Spike S1 Antibody

Expressed Host Mouse MAb
Tag Mouse IgG2a
40150-MM10   SARS-CoV Spike S1 Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40150-T62   SARS-CoV Spike S1 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40150-D001-H   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-D002   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-D003   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-D004   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-D005   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40150-D006   SARS-CoV-2 Spike Antibody

Expressed Host Chimera MAb
Tag rabbit v domain / human IgG1
40150-R007   SARS-CoV-2 Spike Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40589-T62   SARS-CoV-2 Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40591-MM42   SARS-CoV-2 Spike Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40591-T62   SARS-CoV-2 Spike Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40591-MM43   SARS-CoV-2 Spike Neutralizing Antibody

Expressed Host Mouse MAb
Tag Mouse IgG1
40592-MM57   SARS-CoV-2 Spike Neutralizing Antibody

Expressed Host Mouse MAb
Tag Mouse IgG2b
40592-R001   SARS-CoV-2 Spike Neutralizing Antibody

Expressed Host Rabbit MAb
Tag Rabbit IgG
40592-T62   SARS-CoV-2 Spike RBD Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
40590-D001   SARS-CoV-2 Spike S2 Antibody

Expressed Host Chimera MAb
Tag mouse v domain / human IgG1
40590-T62   SARS-CoV-2 Spike S2 Antibody

Expressed Host Rabbit PAb
Tag Rabbit IgG
Recombinant Antibody Production