新型冠状病毒Spike突变体蛋白

最近在英国出现了一种新的SARS-CoV-2毒株。据报道,该突变株的传染力更强,伦敦地区60%的新感染病例均由此毒株引起。CDC将此突变株命名为" SARS-CoV-2 VOC(variant of concern)202012/01",由此突出了其重要的临床意义。

新鉴定的B.1.1.7新冠突变株携带了大量的基因突变,导致了将近14个氨基酸的替换。这其中有8个突变均发生在spike蛋白上,以下3个突变位点尤其引起研究者的关注,并且需要加强对其的监测。
-- N501Y,可增强新冠病毒与人或者鼠ACE2的结合
-- 69-70del,可引起免疫逃逸
-- P681H,靠近furin酶切位点

近日也有报道南非出现新的病毒突变体,同样发现存在N501Y突变。南非卫生部门发现,该突变体在刺突蛋白的受体结合域存在3个位点突变:K417NE484K和N501Y。其中E484K和N501Y属于受体结合基序,是与人ACE2受体形成结合的主要位点。

除了B.1.1.7突变株的这些重点突变体之外,D614G、N439K、Y453F等已经被鉴定的高频突变体也需被列入重点跟踪监测的行列。
-- D614G,可增强病毒传播率
-- N439K,引起免疫逃逸,减弱某些中和抗体的效应
-- Y453F,引起免疫逃逸,减弱某些中和抗体的效应

义翘神州开发了一系列的Spike 突变体蛋白,可用于对新冠突变株的深入研究以及新冠抗体、疫苗效应评价研究。

英国突变株B.1.1.7重要突变位点研究

货号 订购 抗原 突变位点
40592-V08H82 现货 RBD  N501Y 
40591-V08H7 现货 S1  HV69-70 deletion, N501Y, D614G 
40591-V08H12 现货 S1  H69del, V70del, Y144del, N501Y, A570D, D614G, P681H 
40588-V07E7  预定 D3L, R203K, G204R, S235F 
40588-V07E8  预定 D3L, S235F 
40589-V08B6  开发中 S-ECD H69del, V70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H 
Recombinant Spike N501Y Mutant
Cat#: 40592-V08H82
Activity: Bind with ACE2

SARS-CoV-2 Spike RBD Mutant

  • RBD His Tag
    P337S
    40592-V08H62
  • RBD His Tag
    F338L
    40592-V08H26
  • RBD His Tag
    V341I
    40592-V08H11
  • RBD His Tag
    F342L
    40592-V08H6
  • RBD His Tag
    A344S
    40592-V08H37
  • RBD His Tag
    A348S
    40592-V08H25
  • RBD His Tag
    A352S
    40592-V08H58
  • RBD His Tag
    N354D
    40592-V08H2
  • RBD His Tag
    S359N
    40592-V08H32
  • RBD mFc Tag
    V367F
    40592-V05H1
  • RBD His Tag
    V367F
    40592-V08H1
  • RBD His Tag
    N370S
    40592-V08H43
  • RBD His Tag
    A372S
    40592-V08H19
  • RBD His Tag
    A372T
    40592-V08H36
  • RBD His Tag
    F377L
    40592-V08H27
  • RBD His Tag
    K378N
    40592-V08H42
  • RBD His Tag
    K378R
    40592-V08H33
  • RBD His Tag
    P384L
    40592-V08H24
  • RBD His Tag
    T385A
    40592-V08H47
  • RBD His Tag
    T393P
    40592-V08H48
  • RBD His Tag
    V395I
    40592-V08H49
  • RBD His Tag
    D405V, Q414A
    40592-V08H22
  • RBD His Tag
    E406Q
    40592-V08H40
  • RBD His Tag
    R408I
    40592-V08H10
  • RBD His Tag
    Q409E
    40592-V08H34
  • RBD His Tag
    Q414E
    40592-V08H23
  • RBD His Tag
    Q414R
    40592-V08H44
  • RBD His Tag
    K417N
    40592-V08H59
  • RBD His Tag
    A435S
    40592-V08H4
  • RBD His Tag
    W436R
    40592-V08H9
  • RBD His Tag
    N439K
    40592-V08H14
  • RBD His Tag
    N440K
    40592-V08H55
  • RBD His Tag
    K444R
    40592-V08H54
  • RBD His Tag
    V445F
    40592-V08H79
  • RBD His Tag
    G446S
    40592-V08H76
  • RBD His Tag
    G446V
    40592-V08H51
  • RBD His Tag
    L452R
    40592-V08H28
  • RBD His Tag
    Y453F
    40592-V08H80
  • RBD His Tag
    L455F
    40592-V08H68
  • RBD His Tag
    F456E
    40592-V08H73
  • RBD His Tag
    F456L
    40592-V08H71
  • RBD His Tag
    K458Q
    40592-V08H69
  • RBD His Tag
    K458R
    40592-V08H7
  • RBD His Tag
    E471Q
    40592-V08H56
  • RBD His Tag
    I472V
    40592-V08H35
  • RBD His Tag
    G476S
    40592-V08H8
  • RBD His Tag
    S477I
    40592-V08H45
  • RBD His Tag
    S477N
    40592-V08H46
  • RBD His Tag
    S477R
    40592-V08H64
  • RBD His Tag
    T478I
    40592-V08H30
  • RBD His Tag
    P479S
    40592-V08H57
  • RBD His Tag
    N481D
    40592-V08H70
  • RBD His Tag
    G482S
    40592-V08H53
  • RBD His Tag
    V483A
    40592-V08H5
  • RBD His Tag
    V483I
    40592-V08H31
  • RBD His Tag
    E484K
    40592-V08H84
  • RBD His Tag
    E484Q
    40592-V08H81
  • RBD His Tag
    G485S
    40592-V08H52
  • RBD His Tag
    F486S
    40592-V08H74
  • RBD His Tag
    N487R
    40592-V08H75
  • RBD His Tag
    F490L
    40592-V08H83
  • RBD His Tag
    F490S
    40592-V08H41
  • RBD His Tag
    S494P
    40592-V08H18
  • RBD His Tag
    P499R
    40592-V08H78
  • RBD His Tag
    N501Y
    40592-V08H82
  • RBD His Tag
    V503F
    40592-V08H15
  • RBD His Tag
    Y505C
    40592-V08H72
  • RBD His Tag
    Y508H
    40592-V08H12
  • RBD His Tag
    A520S
    40592-V08H20
  • RBD His Tag
    A520V
    40592-V08H39
  • RBD His Tag
    P521R
    40592-V08H63
  • RBD His Tag
    P521S
    40592-V08H29
  • RBD His Tag
    A522S
    40592-V08H21
  • RBD His Tag
    A522V
    40592-V08H16

SARS-CoV-2 Spike S1 Mutant

SARS-CoV-2 Spike S1+S2 ECD Mutant

Recombinant Antibody Production