新冠突变体研究试剂库

近月以来,3株新检测到的具有全球流行性新冠突变株引起了全世界的关注,包括英国B.1.1.7突变株、巴西P.1突变株和南非B.1.351突变株。

在目前已经检测分离到的新冠突变株中,发生在Spike蛋白受体结合域的多个突变是主要的突变类型,也是最令人担心的突变。Spike蛋白受体结合域是新冠病毒结合宿主受体的主要结构,该结构的突变则有可能导致增强与受体的亲和力、减弱中和抗体效应或者造成病毒的免疫逃逸。其中N501Y突变(英国B.1.1.7突变株、巴西P.1突变株和南非B.1.351突变株均有该突变。货号:40592-V08H82)以及E484K突变(巴西P.1突变株和南非B.1.351突变株有该突变。货号:40592-V08H84)尤其受到关注。有研究表明,这两个突变可能和突变株的高传播率有关。

经分析,研究人员发现以上3种新分离的新冠突变株中存在N蛋白突变。N蛋白具有高度保守型,常被用作新冠抗原快速检测的标志物。目前已经上市的新冠抗原检测试剂是否可以检测到突变后的N蛋白以及检测灵敏度和特异性是否会受到突变的影响亟需进一步的研究。

义翘神州开发了80多种重组spike、N抗原突变体蛋白,涵盖了包括上述提及的突变位点在内的多种突变位点。

英国突变株B.1.1.7重要突变位点研究

货号 订购 抗原 突变位点
40592-V08H82 现货 RBD N501Y
40592-V08H82-B 现货 RBD-Biotinylated N501Y
40591-V08H7 现货 S1 HV69-70 deletion, N501Y, D614G
40591-V08H12 现货 S1 H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
40591-V08H12-B 现货 S1-Biotinylated H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
40588-V07E7 现货 N D3L, R203K, G204R, S235F
40588-V07E8 现货 N D3L, S235F
40589-V08B6 现货 S-ECD H69del, V70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H

南非突变株B.1.351重要突变位点研究

货号 订购 抗原 突变位点
40592-V08H82 现货 RBD N501Y
40592-V08H82-B 现货 RBD-Biotinylated N501Y
40592-V08H84 现货 RBD E484K
40592-V08H84-B 现货 RBD-Biotinylated E484K
40592-V08H59 现货 RBD K417N
40592-V08H59-B 现货 RBD-Biotinylated K417N
40592-V08H85 现货 RBD K417N, E484K, N501Y
40592-V08H85-B 现货 RBD-Biotinylated K417N, E484K, N501Y
40591-V08H10 现货 S1 K417N, E484K, N501Y, D614G

巴西突变株P.1重要突变位点研究

货号 订购 抗原 突变位点
40592-V08H82 现货 RBD N501Y
40592-V08H82-B 现货 RBD-Biotinylated N501Y
40592-V08H84 现货 RBD E484K
40592-V08H84-B 现货 RBD-Biotinylated E484K
40592-V08H86 现货 RBD K417T, E484K, N501Y

水貂突变株重要突变位点研究

货号 订购 抗原 突变位点
40592-V08H80 现货 RBD Y453F
40591-V08H8 现货 S1 ΔH69/ΔV70, Y453F, D614G

N蛋白高频突变位点研究

货号 订购 抗原 突变位点
40588-V07E1 现货 N R203K, G204R
40588-V07E2 现货 N P13L
40588-V07E3 现货 N S194L
40588-V07E4 现货 N I292T
40588-V07E9 现货 N T205I
40588-V07E11 现货 N P80R

新冠N蛋白特异性保守区

货号 订购 抗原 结构域
40588-V07E5 现货 N-CTD CTD
40588-V07E10 现货 N-NTD NTD

SARS-CoV-2 Nucleocapsid Mutant

SARS-CoV-2 S1+S2 ECD Mutant

SARS-CoV-2 Spike RBD Mutant

  • RBD His Tag
    P337S
    40592-V08H62
  • RBD His Tag
    F338L
    40592-V08H26
  • RBD His Tag
    V341I
    40592-V08H11
  • RBD His Tag
    F342L
    40592-V08H6
  • RBD His Tag
    A344S
    40592-V08H37
  • RBD His Tag
    A348S
    40592-V08H25
  • RBD His Tag
    A352S
    40592-V08H58
  • RBD His Tag
    N354D
    40592-V08H2
  • RBD His Tag
    S359N
    40592-V08H32
  • RBD mFc Tag
    V367F
    40592-V05H1
  • RBD His Tag
    V367F
    40592-V08H1
  • RBD His Tag
    N370S
    40592-V08H43
  • RBD His Tag
    A372S
    40592-V08H19
  • RBD His Tag
    A372T
    40592-V08H36
  • RBD His Tag
    F377L
    40592-V08H27
  • RBD His Tag
    K378N
    40592-V08H42
  • RBD His Tag
    K378R
    40592-V08H33
  • RBD His Tag
    P384L
    40592-V08H24
  • RBD His Tag
    T385A
    40592-V08H47
  • RBD His Tag
    T393P
    40592-V08H48
  • RBD His Tag
    V395I
    40592-V08H49
  • RBD His Tag
    D405V, Q414A
    40592-V08H22
  • RBD His Tag
    E406Q
    40592-V08H40
  • RBD His Tag
    R408I
    40592-V08H10
  • RBD His Tag
    Q409E
    40592-V08H34
  • RBD His Tag
    Q414E
    40592-V08H23
  • RBD His Tag
    Q414R
    40592-V08H44
  • RBD His Tag
    K417N
    40592-V08H59
  • RBD His Tag
    K417N
    40592-V08H59-B
  • RBD His Tag
    K417N, E484K, N501Y
    40592-V08H85
  • RBD His Tag
    K417N, E484K, N501Y
    40592-V08H85-B
  • RBD His Tag
    K417T, E484K, N501Y
    40592-V08H86
  • RBD His Tag
    A435S
    40592-V08H4
  • RBD His Tag
    W436R
    40592-V08H9
  • RBD His Tag
    N439K
    40592-V08H14
  • RBD His Tag
    N440K
    40592-V08H55
  • RBD His Tag
    K444R
    40592-V08H54
  • RBD His Tag
    V445F
    40592-V08H79
  • RBD His Tag
    G446S
    40592-V08H76
  • RBD His Tag
    G446V
    40592-V08H51
  • RBD His Tag
    L452R
    40592-V08H28
  • RBD His Tag
    Y453F
    40592-V08H80
  • RBD His Tag
    L455F
    40592-V08H68
  • RBD His Tag
    F456E
    40592-V08H73
  • RBD His Tag
    F456L
    40592-V08H71
  • RBD His Tag
    K458Q
    40592-V08H69
  • RBD His Tag
    K458R
    40592-V08H7
  • RBD His Tag
    E471Q
    40592-V08H56
  • RBD His Tag
    I472V
    40592-V08H35
  • RBD His Tag
    G476S
    40592-V08H8
  • RBD His Tag
    S477I
    40592-V08H45
  • RBD His Tag
    S477N
    40592-V08H46
  • RBD His Tag
    S477R
    40592-V08H64
  • RBD His Tag
    T478I
    40592-V08H30
  • RBD His Tag
    P479S
    40592-V08H57
  • RBD His Tag
    N481D
    40592-V08H70
  • RBD His Tag
    G482S
    40592-V08H53
  • RBD His Tag
    V483A
    40592-V08H5
  • RBD His Tag
    V483I
    40592-V08H31
  • RBD His Tag
    E484K
    40592-V08H84
  • RBD His Tag
    E484K
    40592-V08H84-B
  • RBD His Tag
    E484Q
    40592-V08H81
  • RBD His Tag
    G485S
    40592-V08H52
  • RBD His Tag
    F486S
    40592-V08H74
  • RBD His Tag
    N487R
    40592-V08H75
  • RBD His Tag
    F490L
    40592-V08H83
  • RBD His Tag
    F490S
    40592-V08H41
  • RBD His Tag
    S494P
    40592-V08H18
  • RBD His Tag
    P499R
    40592-V08H78
  • RBD His Tag
    N501Y
    40592-V08H82
  • RBD His Tag
    N501Y
    40592-V08H82-B
  • RBD Fc Tag
    N501Y
    40592-V02H1
  • RBD His Tag
    V503F
    40592-V08H15
  • RBD His Tag
    Y505C
    40592-V08H72
  • RBD His Tag
    Y508H
    40592-V08H12
  • RBD His Tag
    A520S
    40592-V08H20
  • RBD His Tag
    A520V
    40592-V08H39
  • RBD His Tag
    P521R
    40592-V08H63
  • RBD His Tag
    P521S
    40592-V08H29
  • RBD His Tag
    A522S
    40592-V08H21
  • RBD His Tag
    A522V
    40592-V08H16

SARS-CoV-2 Spike S1 Mutant

SARS-CoV-2 Spike S1+S2 ECD Mutant

Recombinant Antibody Production