ACE2 Antibody Guideline: Preparation, Dilution and Applications

ACE2 Antibody Overview

There are 15 ACE2 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, FCM, Neutralization, WB, IP, ELISA(Cap), ELISA(Det). There are 9 ACE2 antibody for ELISA, 5 ACE2 antibody for IHC-P, 6 ACE2 antibody for FCM, 2 ACE2 antibody for Neutralization, 1 ACE2 antibody for WB, 1 ACE2 antibody for IP, 1 ACE2 antibody for ELISA(Cap), 1 ACE2 antibody for ELISA(Det). Among all these ACE2 antibodies, there are 7 anti-ACE2 mouse monoclonal antibodies , 4 anti-ACE2 rabbit monoclonal antibodies , 4 anti-ACE2 rabbit polyclonal antibodies . All the ACE2 anbodies are produced in house and all are in stock. ACE2 antibody customerized service is available.

ACE2 Antibody ELISA

ACE2 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 (rh ACE2 / Angiotensin-Converting Enzyme 2; NP_068576.1; 1M-740S). Total IgG was purified by Protein A affinity chromatography.

ACE2 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

ACE2 Antibody western blot

ACE2 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 (rh ACE2 / Angiotensin-Converting Enzyme 2; NP_068576.1; 1M-740S). ACE2 / Angiotensin-Converting Enzyme 2 specific IgG was purified by Human ACE2 / Angiotensin-Converting Enzyme 2 affinity chromatography.

ACE2 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

ACE2 Antibody immunohistochemistry

ACE2 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Rat ACE2 (rR ACE2; Catalog#80031-R08H; Q5EGZ1; Met1-Thr740).

ACE2 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

ACE2 antibody citations

Title
Evidence for gastrointestinal infection of SARS-CoV-2
Year
2020
Author
Xiao, F;Tang, M;Zheng, X;Liu, Y;Li, X;Shan, H;
Journal
Gastroenterology
Title
Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids
Year
2020
Author
Zhao, B;Ni, C;Gao, R;Wang, Y;Yang, L;Wei, J;Lv, T;
Journal
bioRxiv
Title
The Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Directly Decimates Human Spleens and Lymph Nodes
Year
2020
Author
Feng, Z;Diao, B;Wang, R;Wang, G;Wang, C;Tan, Y;
Journal
medRxiv
Title
Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2
Year
2020
Author
Yao, D;Lao, F;Zhang, Z;Liu, Y;Cheng, J;Ding, F;Wang, X;Xi, L;Wang, C;Yan, X;Zhang, R;Ouyang, F;Ding, H;Ke, T;
Journal
bioRxiv