ADAM17 Antibody Guideline: Preparation, Dilution and Applications

ADAM17 Antibody Overview

There are 4 ADAM17 antibodies which are validated in multiple tissues with various applications, including IP, WB, ICC/IF, IHC-P, ELISA. There are 1 ADAM17 antibody for IP, 1 ADAM17 antibody for WB, 1 ADAM17 antibody for ICC/IF, 1 ADAM17 antibody for IHC-P, 1 ADAM17 antibody for ELISA. Among all these ADAM17 antibodies, there are 4 anti-ADAM17 rabbit polyclonal antibodies . All the ADAM17 anbodies are produced in house and all are in stock. ADAM17 antibody customerized service is available.

ADAM17 Antibody ELISA

ADAM17 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Rat ADAM17 (Catalog#80350-R08H; Q9Z1K9; Met1-Asp563). ADAM17 specific IgG was purified by Rat ADAM17 affinity chromatography.

ADAM17 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

ADAM17 Antibody western blot

ADAM17 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ADAM17, and purified by antigen affinity chromatography.

ADAM17 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

ADAM17 Antibody immunoprecipitation

ADAM17 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human CD156b, and purified by antigen affinity chromatography.

ADAM17 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

ADAM17 Antibody ICC / IF

ADAM17 Antibody ICC / IF Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ADAM17, and purified by antigen affinity chromatography.

ADAM17 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes