Aminoacylase 1 Antibody Guideline: Preparation, Dilution and Applications

Aminoacylase 1 Antibody Overview

There are 9 Aminoacylase 1 antibodies which are validated in multiple tissues with various applications, including WB, ELISA, IHC-P, FCM, ICC/IF, IP. There are 3 Aminoacylase 1 antibody for WB, 5 Aminoacylase 1 antibody for ELISA, 3 Aminoacylase 1 antibody for IHC-P, 2 Aminoacylase 1 antibody for FCM, 1 Aminoacylase 1 antibody for ICC/IF, 2 Aminoacylase 1 antibody for IP. Among all these Aminoacylase 1 antibodies, there are 2 anti-Aminoacylase 1 mouse monoclonal antibodies , 3 anti-Aminoacylase 1 rabbit monoclonal antibodies , 4 anti-Aminoacylase 1 rabbit polyclonal antibodies . All the Aminoacylase 1 anbodies are produced in house and all are in stock. Aminoacylase 1 antibody customerized service is available.

Aminoacylase 1 Antibody ELISA

Aminoacylase 1 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human ACY1 (rh ACY1; Catalog#10549-H08B; NP_000657.1; Met 1-Ser 408). Total IgG was purified by Protein A affinity chromatography .

Aminoacylase 1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Aminoacylase 1 Antibody western blot

Aminoacylase 1 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 (rh ACY1; Catalog#10549-H08B; NP_000657.1; Met 1-Ser 408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Aminoacylase 1 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Aminoacylase 1 Antibody flow cytometry

Aminoacylase 1 Antibody immunohistochemistry

Aminoacylase 1 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 / Aminoacylase-1 (rh ACY1 / Aminoacylase-1; Catalog#10549-H08B; NP_000657.1; Met1-Ser408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Aminoacylase 1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

Aminoacylase 1 Antibody immunoprecipitation

Aminoacylase 1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse Aminoacylase-1 / ACY1 (rM Aminoacylase-1 / ACY1; Catalog#50008-M08H; Q99JW2; Met1-Ser408). Aminoacylase-1 / ACY1 specific IgG was purified by Mouse Aminoacylase-1 / ACY1 affinity chromatography.

Aminoacylase 1 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

Aminoacylase 1 Antibody ICC / IF

Aminoacylase 1 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human ACY1 / Aminoacylase-1 (rh ACY1 / Aminoacylase-1; Catalog#10549-H08B; NP_000657.1; Met1-Ser408).

Aminoacylase 1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes