Angiotensinogen Antibody Guideline: Preparation, Dilution and Applications

Angiotensinogen Antibody Overview

There are 11 Angiotensinogen antibodies which are validated in multiple tissues with various applications, including ELISA(Cap), FCM, ICC/IF, WB, ELISA, IP, IHC-P. There are 1 Angiotensinogen antibody for ELISA(Cap), 4 Angiotensinogen antibody for FCM, 2 Angiotensinogen antibody for ICC/IF, 3 Angiotensinogen antibody for WB, 5 Angiotensinogen antibody for ELISA, 1 Angiotensinogen antibody for IP, 2 Angiotensinogen antibody for IHC-P. Among all these Angiotensinogen antibodies, there are 3 anti-Angiotensinogen mouse monoclonal antibodies , 4 anti-Angiotensinogen rabbit monoclonal antibodies , 4 anti-Angiotensinogen rabbit polyclonal antibodies . All the Angiotensinogen anbodies are produced in house and all are in stock. Angiotensinogen antibody customerized service is available.

Angiotensinogen Antibody ELISA

Angiotensinogen Antibody western blot

Angiotensinogen Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse Angiotensinogen / SerpinA8 / AGT (rM Angiotensinogen / SerpinA8 / AGT; Catalog#50337-M08H; P11859; Met 1-Val 477). Angiotensinogen / SerpinA8 / AGT specific IgG was purified by Mouse Angiotensinogen / SerpinA8 / AGT affinity chromatography.

Angiotensinogen Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Angiotensinogen Antibody flow cytometry

Angiotensinogen Antibody immunohistochemistry

Angiotensinogen Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human SerpinA8 / angiotensinogen (rh SerpinA8; Catalog#10994-H08H; NP_000020.1; Met 1-Ala 485). SerpinA8 specific IgG was purified by human Serpin A8 affinity chromatography.

Angiotensinogen Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

Angiotensinogen Antibody immunoprecipitation

Angiotensinogen Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human SerpinA8 / angiotensinogen (rh SerpinA8; Catalog#10994-H08H; NP_000020.1; Met 1-Ala 485).

Angiotensinogen Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

Angiotensinogen Antibody ICC / IF

Angiotensinogen Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinA8 / Angiotensinogen / AGT (rh SerpinA8 / Angiotensinogen / AGT; Catalog#10994-H08H; NP_000020.1; Met 1-Ala 485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Angiotensinogen Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes