Custom Antibody FAQs

The following are frequently asked questions about our custom antibody production and development services.

  • What is included in one-stop-shop antibody services package?

    One-stop-shop antibody service package includes antigen design, immunization and screening, antibody subtype identification, antibody gene cloning, antibody production, antibody purification and quality control.

  • What are the milestones and what is the timeline?

    The main milestones are antigen production, antibody development and antibody production. The timeline for peptide design, synthesis and conjugation is 4-6 weeks. The timeline for antigen protein production depends on the chosen expression system and the difficulties to produce. Please refer to protein production service section for details. The timeline for polyclonal antibody development is 9-12 weeks. The timeline for mouse/rabbit monoclonal antibody development is 4-6 months. The timeline for antibody production is 4-8 weeks, depending on the required quantity.

  • Do I get timely updates?

    Sino Biological has established an efficient and smooth project management practice with a minimum required involvement from clients. This type of partnership allows clients the flexibility to dedicate and decide the degree of involvement in the project, and hence cutting overall overhead and project management costs. Clients get timely updates if there are any problems which could cause delays.

  • What information or material do I need to provide to Sino Biological and what deliverables do I get? Do I get the hybridoma clones?

    Please provide the information of the antigen and describe the target quality specifications of the final product. Please refer to the request form for the required information. The deliverable is customized and it can be purified antibodies, hybridoma cells or antibody sequences.

  • I want to send plasmids to Sino Biological. How do I do?

    Please prepare plasmids according to the following protocol for keeping plasmid in FTA elute card (Whatman Cat. No.: WB120410, WB120401, WB120461) at room temperature.
    HANDLING: Always wear gloves to avoid contamination of FTA Elute Cards.
    1). Label the FTA Elute Card with the appropriate sample identification. Use one card for each biological sample.
    2). Pipet the biological sample (40μl per 11 mm circle) onto the card in a concentric circular motion within the printed circle area slowly.
    3). Samples applied to FTA Elute Cards are ready for immediate room temperature storage. Note: If samples are to be processed shortly after application on the FTA Elute Card, allow the sample to dry completely for at least 3 hours at room temperature prior to punching. To reduce drying time, spotted FTA Elute cards can be placed at 80°C for 15-20 minutes.

  • What is the payment term?

    For pilot studies to access the feasibility and yield, full upfront payment is required. For the rest, 50% upfront payment is required. After the receipt of the products, the rest of the payment is required within one month.

  • Do I get a refund if the project fails?

    Projects are typically defined with multiple milestones. Sino Biological will charge the successful milestones only. If the upfront payment exceeds the actual cost occurred, then the remaining will be refunded to you.

    For example, in antibody production projects, if antibody expression yield is too low or prone to aggregation, a revised price quotation will be provided after the initial assessment of productivity. You have the option to reject the revised pricing and terminate the project with a minimum evaluation fee of $500, plus cost for gene synthesis and vector construction. If the total charge is greater than your upfront payment, we will send you back a refund within 30-days. We have an extremely high success rate for antibody production, so this rarely happens.

  • I want to get antibodies that are applicable for FCM or IHC, could Sino Biological provide the service?

    Currently, Sino Biological does not provide FCM or IHC guaranteed antibody development services. However, clients could choose FCM or IHC service package which include extra FCM or IHC quality control analysis instead of standard ELISA assay. It is recommended that multiple antibody clones could be ordered for your FCM or IHC analysis.

  • What purity and concentration of the final product does Sino Biological usually provide?

    The final purity and concentration differs from product to product according to client's final requirement.

  • Can I have customized concentration and formulation?

    Yes. Client could require customized final concentration and formulation according to downstream work. New proposals will be provided if there are any problems with the required concentration and formulation.

  • Can I get the antibody expression vector as part of deliverables?

    The antibody expression vector used at Sino Biological is optimized and confidential. A commercial expression vector could be provided as part of deliverables according to your requirement.

  • How do I Submit a Request for Antibody Production Services?

    If you are interested in using our antibody production services to support your antibody discovery and development program, please contact us by leaving an online message or send us your detailed requests to [email protected] Based on the quantity and quality requirement of your requests, a formal quote will be sent back to you for your review, comment, and/or acceptance.

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