beta-Actin Antibody Guideline: Preparation, Dilution and Applications

beta-Actin Antibody Overview

There are 2 beta-Actin antibodies which are validated in multiple tissues with various applications, including WB, IP. There are 2 beta-Actin antibody for WB, 1 beta-Actin antibody for IP. Among all these beta-Actin antibodies, there are 1 anti-beta-Actin mouse monoclonal antibodies , 1 anti-beta-Actin rabbit polyclonal antibodies . All the beta-Actin anbodies are produced in house and all are in stock. beta-Actin antibody customerized service is available.

beta-Actin Antibody western blot

beta-Actin Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

beta-Actin Antibody western blot Validated

Application WB
Dilution 1:2500-1:200000
Notes  

beta-Actin Antibody immunoprecipitation

beta-Actin Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB, and purified by antigen affinity chromatography.

beta-Actin Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 uL/mg of lysate
Notes  

beta-Actin antibody citations

Title
dalbinol通过ROS/Dvl/GSK-3β/β-catenin信号通路诱导人结肠癌细胞凋亡的作用机制研究
Year
2016
Author
LI, F;XinWU;LIAO, H;QIU, S;ZHU, X;CUI, L;WU, H;
Journal
Advanced Materials Interfaces
Title
Manipulations in HIWI level exerts influence on the proliferation of human non-small cell lung cancer cells
Year
2016
Author
Wang, Y;Liu, J;Wu, G;Yang, F;
Journal
Exp Ther Med
Title
MicroRNA-495 regulates starvation-induced autophagy by targeting ATG3
Year
2016
Author
Li, W;Yang, Y;Hou, X;Zhuang, H;Wu, Z;Li, Z;Guo, R;Chen, H;Lin, C;Zhong, W;Chen, Y;Wu, K;Zhang, L;Feng, D;
Journal
FEBS Lett.
Title
Inhibition of ANO1/TMEM16A induces apoptosis in human prostate carcinoma cells by activating TNF-α signaling
Year
2018
Author
Song, Y;Gao, J;Guan, L;Chen, X;Gao, J;Wang, K;
Journal
Cell Death Dis