Beta-Tubulin Antibody Guideline: Preparation, Dilution and Applications

Beta-Tubulin Antibody Overview

There are 1 Beta-Tubulin antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ICC/IF, IP. There are 1 Beta-Tubulin antibody for WB, 1 Beta-Tubulin antibody for IHC-P, 1 Beta-Tubulin antibody for ICC/IF, 1 Beta-Tubulin antibody for IP. Among all these Beta-Tubulin antibodies, there are 1 anti-Beta-Tubulin mouse monoclonal antibodies . All the Beta-Tubulin anbodies are produced in house and all are in stock. Beta-Tubulin antibody customerized service is available.

Beta-Tubulin Antibody western blot

Beta-Tubulin Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Beta-Tubulin Antibody western blot Validated

Application WB
Dilution 1/1000-1/100000
Notes  

Beta-Tubulin Antibody immunohistochemistry

Beta-Tubulin Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Beta-Tubulin Antibody immunohistochemistry Validated

Application IHC
Dilution 1/2000-1/20000
Notes  

Beta-Tubulin Antibody immunoprecipitation

Beta-Tubulin Antibody immunoprecipitation Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Beta-Tubulin Antibody immunoprecipitation Validated

Application IP
Dilution IP:5-10 μL/mg of lysate
Notes  

Beta-Tubulin Antibody ICC / IF

Beta-Tubulin Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Beta-Tubulin Antibody ICC / IF Validated

Application ICC_IF
Dilution 1/50-1/200
Notes  

Beta-Tubulin antibody citations

Title
Amyloid precursor-like protein 2 C-terminal fragments upregulate S100A9 gene and protein expression in BV2 cells
Year
2014
Author
Li, G;Chen, H;Cheng, L;Zhao, R;Zhao, J;Xu, Y;
Journal
Neural Regen Res
Title
Design, synthesis and biological evaluation of shikonin thio-glycoside derivatives: new anti-tubulin agents
Year
2014
Author
HY Lin, HW Han, LF Bai, HY Qiu, DZ Yin, JL Qi…
Journal
RSC Advances
Title
Reelin regulates male mouse reproductive capacity via the sertoli cells
Year
2018
Author
Zhuang, M;Li, B;Huang, Y;Lei, Q;Yan, R;Li, N;Sidhu, K;Cheng, X;Yan, X;Miao, Y;Zhao, S;Hua, J;
Journal
J. Cell. Biochem.
Title
ATG3, a Target of miR-431-5p, Promotes Proliferation and Invasion of Colon Cancer via Promoting Autophagy
Year
2019
Author
Huang, W;Zeng, C;Hu, S;Wang, L;Liu, J;
Journal
Cancer Manag Res
Title
Long-chain fatty acyl-coenzyme A suppresses hepatitis C virus infection by targeting virion-bound lipoproteins
Year
2020
Author
Li, X;Li, J;Feng, Y;Cai, H;Li, YP;Peng, T;
Journal
Antiviral Res.