BID Antibody Guideline: Preparation, Dilution and Applications

BID Antibody Overview

There are 12 BID antibodies which are validated in multiple tissues with various applications, including WB, ELISA, ELISA(Det), ELISA(Cap), FCM, ICC/IF, IP, IHC-P. There are 7 BID antibody for WB, 9 BID antibody for ELISA, 1 BID antibody for ELISA(Det), 1 BID antibody for ELISA(Cap), 3 BID antibody for FCM, 2 BID antibody for ICC/IF, 5 BID antibody for IP, 2 BID antibody for IHC-P. Among all these BID antibodies, there are 5 anti-BID mouse monoclonal antibodies , 2 anti-BID rabbit monoclonal antibodies , 5 anti-BID rabbit polyclonal antibodies . All the BID anbodies are produced in house and all are in stock. BID antibody customerized service is available.

BID Antibody ELISA

BID antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met 1-Asp 195). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BID antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

BID Antibody western blot

BID Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met1-Asp195). Total IgG was purified by Protein A affinity chromatography.

BID Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

BID Antibody flow cytometry

BID Antibody immunohistochemistry

BID Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human p22 BID (rh p22 BID; Catalog#10468-HNCE; P55957-1; Met1-Asp195). p22 BID specific IgG was purified by Human p22 BID affinity chromatography.

BID Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:3000
Notes  

BID Antibody immunoprecipitation

BID Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met1-Asp195).

BID Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-2 μL/mg of lysate
Notes  

BID Antibody ICC / IF

BID Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met 1-Asp 195). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BID Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes