Carbonic Anhydrase III Antibody Guideline: Preparation, Dilution and Applications

Carbonic Anhydrase III Antibody Overview

There are 6 Carbonic Anhydrase III antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, WB, IP. There are 3 Carbonic Anhydrase III antibody for ELISA, 2 Carbonic Anhydrase III antibody for IHC-P, 2 Carbonic Anhydrase III antibody for WB, 2 Carbonic Anhydrase III antibody for IP. Among all these Carbonic Anhydrase III antibodies, there are 1 anti-Carbonic Anhydrase III mouse monoclonal antibodies , 2 anti-Carbonic Anhydrase III rabbit monoclonal antibodies , 3 anti-Carbonic Anhydrase III rabbit polyclonal antibodies . All the Carbonic Anhydrase III anbodies are produced in house and all are in stock. Carbonic Anhydrase III antibody customerized service is available.

Carbonic Anhydrase III Antibody ELISA

Carbonic Anhydrase III antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; Met 1-Lys 260; NP_005172.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Carbonic Anhydrase III antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Carbonic Anhydrase III Antibody western blot

Carbonic Anhydrase III Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; NP_005172.1; Met1-Lys260).

Carbonic Anhydrase III Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Carbonic Anhydrase III Antibody immunohistochemistry

Carbonic Anhydrase III Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; Met 1-Lys 260; NP_005172.1). Carbonic Anhydrase III / CA3 specific IgG was purified by Human Carbonic Anhydrase III / CA3 affinity chromatography.

Carbonic Anhydrase III Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

Carbonic Anhydrase III Antibody immunoprecipitation

Carbonic Anhydrase III Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Carbonic Anhydrase III/CA3, and purified by antigen affinity chromatography.

Carbonic Anhydrase III Antibody immunoprecipitation Validated

Application IP
Dilution IP:5-10 μL/mg of lysate
Notes