Carbonic Anhydrase IV Antibody Guideline: Preparation, Dilution and Applications

Carbonic Anhydrase IV Antibody Overview

There are 5 Carbonic Anhydrase IV antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, WB, IP. There are 2 Carbonic Anhydrase IV antibody for ELISA, 4 Carbonic Anhydrase IV antibody for IHC-P, 2 Carbonic Anhydrase IV antibody for WB, 1 Carbonic Anhydrase IV antibody for IP. Among all these Carbonic Anhydrase IV antibodies, there are 1 anti-Carbonic Anhydrase IV rabbit monoclonal antibodies , 4 anti-Carbonic Anhydrase IV rabbit polyclonal antibodies . All the Carbonic Anhydrase IV anbodies are produced in house and all are in stock. Carbonic Anhydrase IV antibody customerized service is available.

Carbonic Anhydrase IV Antibody ELISA

Carbonic Anhydrase IV antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 (Catalog#50350-M08H; NP_031633.1; Met1-Ser277). Total IgG was purified by Protein A affinity chromatography.

Carbonic Anhydrase IV antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Carbonic Anhydrase IV Antibody western blot

Carbonic Anhydrase IV Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 (Catalog#50350-M08H; NP_031633.1; Met1-Ser277). Carbonic Anhydrase IV / Car4 / CA4 specific IgG was purified by Mouse Carbonic Anhydrase IV / Car4 / CA4 affinity chromatography.

Carbonic Anhydrase IV Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Carbonic Anhydrase IV Antibody immunohistochemistry

Carbonic Anhydrase IV Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV / CA4 (rh Carbonic Anhydrase IV / CA4; Catalog#10472-H08H; NP_000708.1; Met1-Lys283).

Carbonic Anhydrase IV Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

Carbonic Anhydrase IV Antibody immunoprecipitation

Carbonic Anhydrase IV Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase IV / CA4 (rh Carbonic Anhydrase IV / CA4; Catalog#10472-H08H; NP_000708.1; Met 1-Lys 283). Carbonic Anhydrase IV / CA4 specific IgG was purified by Human Carbonic Anhydrase IV / CA4 affinity chromatography.

Carbonic Anhydrase IV Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes