Carbonic Anhydrase IX Antibody Guideline: Preparation, Dilution and Applications

Carbonic Anhydrase IX Antibody Overview

There are 16 Carbonic Anhydrase IX antibodies which are validated in multiple tissues with various applications, including ELISA, WB, IP, IHC-P, FCM. There are 12 Carbonic Anhydrase IX antibody for ELISA, 3 Carbonic Anhydrase IX antibody for WB, 2 Carbonic Anhydrase IX antibody for IP, 4 Carbonic Anhydrase IX antibody for IHC-P, 3 Carbonic Anhydrase IX antibody for FCM. Among all these Carbonic Anhydrase IX antibodies, there are 1 anti-Carbonic Anhydrase IX mouse monoclonal antibodies , 8 anti-Carbonic Anhydrase IX rabbit monoclonal antibodies , 7 anti-Carbonic Anhydrase IX rabbit polyclonal antibodies . All the Carbonic Anhydrase IX anbodies are produced in house and all are in stock. Carbonic Anhydrase IX antibody customerized service is available.

Carbonic Anhydrase IX Antibody ELISA

Carbonic Anhydrase IX antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase IX/CA9 (rh Carbonic Anhydrase IX/CA9; Catalog#10107-H08H; NP_001207.2; Met1-Asp414). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Carbonic Anhydrase IX antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Carbonic Anhydrase IX Antibody western blot

Carbonic Anhydrase IX Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; Catalog#10107-H08H; NP_001207.2; Met1-Asp414). Carbonic Anhydrase IX / CA9 specific IgG was purified by Human Carbonic Anhydrase IX / CA9 affinity chromatography.

Carbonic Anhydrase IX Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Carbonic Anhydrase IX Antibody flow cytometry

Carbonic Anhydrase IX Antibody immunohistochemistry

Carbonic Anhydrase IX Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; Catalog#10107-H08H; NP_001207.2; Met 1-Asp 414).

Carbonic Anhydrase IX Antibody immunohistochemistry Validated

Application IHC
Dilution 1:250-1:1000
Notes  

Carbonic Anhydrase IX Antibody immunoprecipitation

Carbonic Anhydrase IX Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human carbonic anhydrase IX (rhCAIX; Catalog#10107-H08H; NP_001207.2; Met 1-Asp 414).

Carbonic Anhydrase IX Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

Carbonic Anhydrase IX antibody citations

Title
Fabrication of mAb G250-SPIO molecular magnetic resonance imaging nanoprobe for the specific detection of renal cell carcinoma in vitro
Year
2014
Author
Lu, C;Li, J;Xu, K;Yang, C;Wang, J;Han, C;Liu, X;
Journal
PLoS ONE