CCR2 Antibody Guideline: Preparation, Dilution and Applications

CCR2 Antibody Overview

There are 6 CCR2 antibodies which are validated in multiple tissues with various applications, including FCM, WB, ICC/IF, IP. There are 4 CCR2 antibody for FCM, 2 CCR2 antibody for WB, 1 CCR2 antibody for ICC/IF, 1 CCR2 antibody for IP. Among all these CCR2 antibodies, there are 4 anti-CCR2 mouse monoclonal antibodies , 2 anti-CCR2 rabbit polyclonal antibodies . All the CCR2 anbodies are produced in house and all are in stock. CCR2 antibody customerized service is available.

CCR2 Antibody western blot

CCR2 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human CCR2/CD192, and purified by antigen affinity chromatography.

CCR2 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CCR2 Antibody flow cytometry

CCR2 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human CCR2/CD192. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CCR2 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CCR2 Antibody immunoprecipitation

CCR2 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CCR2/cd192, and purified by antigen affinity chromatography.

CCR2 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

CCR2 Antibody ICC / IF

CCR2 Antibody ICC / IF Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CCR2/CD192, and purified by antigen affinity chromatography.

CCR2 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes