CD13 Antibody Guideline: Preparation, Dilution and Applications

CD13 Antibody Overview

There are 14 CD13 antibodies which are validated in multiple tissues with various applications, including ELISA, WB, IHC-P, IP, ICC/IF. There are 8 CD13 antibody for ELISA, 6 CD13 antibody for WB, 9 CD13 antibody for IHC-P, 3 CD13 antibody for IP, 1 CD13 antibody for ICC/IF. Among all these CD13 antibodies, there are 3 anti-CD13 mouse monoclonal antibodies , 5 anti-CD13 rabbit monoclonal antibodies , 6 anti-CD13 rabbit polyclonal antibodies . All the CD13 anbodies are produced in house and all are in stock. CD13 antibody customerized service is available.

CD13 Antibody ELISA

CD13 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; Catalog#10051-H08H; NP_001141.2; Lys69-Lys967,603Met/Ile). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD13 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD13 Antibody western blot

CD13 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; Catalog#10051-H08H; NP_001141.2; Lys 69-Lys 967, 603 Met/Ile).

CD13 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD13 Antibody immunohistochemistry

CD13 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; Catalog#10051-H08H; NP_001141.2; Lys69-Lys967,603Met/Ile). CD13 / ANPEP specific IgG was purified by Human CD13 / ANPEP affinity chromatography.

CD13 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:5000-1:20000
Notes  

CD13 Antibody immunoprecipitation

CD13 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human ANPEP (rh ANPEP; Catalog#10051-H08H; NP_001141.2; Lys69-Lys967,603Met/Ile). ANPEP specific IgG was purified by human ANPEP affinity chromatography .

CD13 Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-1 μL/mg of lysate
Notes  

CD13 Antibody ICC / IF

CD13 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human CD13/ANPEP (rh CD13/ANPEP; Catalog#10051-H08H; NP_001141.2; Lys69-Lys967,603Met/Ile). CD13/ANPEP specific IgG was purified by Human CD13/ANPEP affinity chromatography.

CD13 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes