CD14 Antibody Guideline: Preparation, Dilution and Applications

CD14 Antibody Overview

There are 40 CD14 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, IHC-P, ICC/IF, WB, ELISA(Det), ELISA(Cap). There are 13 CD14 antibody for ELISA, 22 CD14 antibody for FCM, 5 CD14 antibody for IHC-P, 2 CD14 antibody for ICC/IF, 1 CD14 antibody for WB, 1 CD14 antibody for ELISA(Det), 1 CD14 antibody for ELISA(Cap). Among all these CD14 antibodies, there are 15 anti-CD14 mouse monoclonal antibodies , 14 anti-CD14 rabbit monoclonal antibodies , 11 anti-CD14 rabbit polyclonal antibodies . All the CD14 anbodies are produced in house and all are in stock. CD14 antibody customerized service is available.

CD14 Antibody ELISA

CD14 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD14 (rh CD14; Catalog#10073-H08H; NP_000582.1; Met 1-Met 344). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD14 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD14 Antibody western blot

CD14 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 extracellular domain (rM CD14; Catalog#50023-M03H; NP_033971.1; Met 1-Pro 345).

CD14 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD14 Antibody flow cytometry

CD14 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD14 (rh CD14; Catalog#10073-H08H; NP_000582.1; Met1-Met344). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD14 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD14 Antibody immunohistochemistry

CD14 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD14 (rh CD14; Catalog#10073-H08H; NP_000582.1; Met1-Met344). CD14 specific IgG was purified by Human CD14 affinity chromatography.

CD14 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CD14 Antibody ICC / IF

CD14 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 (rM CD14; Catalog#50023-M08H; NP_033971.1; Met1-Pro345).

CD14 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD14 antibody citations

Title
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Year
2017
Author
Sung, SJ;Ge, Y;Dai, C;Wang, H;Fu, SM;Sharma, R;Hahn, YS;Yu, J;Le, TH;Okusa, MD;Bolton, WK;Lawler, JR;
Journal
J. Immunol
Title
Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis
Year
2019
Author
Kuriakose, J;Redecke, V;Guy, C;Zhou, J;Wu, R;Ippagunta, SK;Tillman, H;Walker, PD;Vogel, P;Häcker, H;
Journal
J. Clin. Invest.