CD147 Antibody Guideline: Preparation, Dilution and Applications

CD147 Antibody Overview

There are 16 CD147 antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Cap), IHC-P, FCM, ICC/IF, ELISA(Det), WB, IHC-Fr, IP. There are 9 CD147 antibody for ELISA, 1 CD147 antibody for ELISA(Cap), 3 CD147 antibody for IHC-P, 8 CD147 antibody for FCM, 5 CD147 antibody for ICC/IF, 1 CD147 antibody for ELISA(Det), 2 CD147 antibody for WB, 1 CD147 antibody for IHC-Fr, 1 CD147 antibody for IP. Among all these CD147 antibodies, there are 5 anti-CD147 mouse monoclonal antibodies , 7 anti-CD147 rabbit monoclonal antibodies , 4 anti-CD147 rabbit polyclonal antibodies . All the CD147 anbodies are produced in house and all are in stock. CD147 antibody customerized service is available.

CD147 gene family antibody

CD147 Antibody ELISA

CD147 Antibody western blot

CD147 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD147. (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). Total IgG was purified by Protein A affinity chromatography.

CD147 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD147 Antibody flow cytometry

CD147 Antibody immunohistochemistry

CD147 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD147. (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD147 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD147 Antibody immunoprecipitation

CD147 Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD147 / Basigin (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205).

CD147 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

CD147 Antibody ICC / IF

CD147 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD147 / EMMPRIN / Basigin (rh CD147 / EMMPRIN / Basigin; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD147 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD147 antibody citations

Title
Molecular association of CD98, CD29, and CD147 critically mediates monocytic U937 cell adhesion
Year
2016
Author
Kim, MY;Cho, JY;
Journal
Korean J. Physiol. Pharmacol.