CD166/ALCAM Antibody Guideline: Preparation, Dilution and Applications

CD166/ALCAM Antibody Overview

There are 24 CD166/ALCAM antibodies which are validated in multiple tissues with various applications, including FCM, ICC/IF, ELISA, WB, IHC-P, IP. There are 11 CD166/ALCAM antibody for FCM, 1 CD166/ALCAM antibody for ICC/IF, 13 CD166/ALCAM antibody for ELISA, 5 CD166/ALCAM antibody for WB, 3 CD166/ALCAM antibody for IHC-P, 2 CD166/ALCAM antibody for IP. Among all these CD166/ALCAM antibodies, there are 5 anti-CD166/ALCAM mouse monoclonal antibodies , 10 anti-CD166/ALCAM rabbit polyclonal antibodies , 9 anti-CD166/ALCAM rabbit monoclonal antibodies . All the CD166/ALCAM anbodies are produced in house and all are in stock. CD166/ALCAM antibody customerized service is available.

CD166/ALCAM gene family antibody

CD166/ALCAM Antibody ELISA

CD166/ALCAM antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166 / ALCAM (rh CD166 / ALCAM; Catalog#10045-H08H; NP_001618.2; Met 1-Ala 526). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD166/ALCAM antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD166/ALCAM Antibody western blot

CD166/ALCAM Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD166 / ALCAM (Catalog#90227-C08H; G7NZQ8; Met1-Ala526).

CD166/ALCAM Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD166/ALCAM Antibody flow cytometry

CD166/ALCAM Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD166 / ALCAM (rM CD166 / ALCAM; Catalog#50005-M08H; NP_033785.1; Met1-Lys527).

CD166/ALCAM Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD166/ALCAM Antibody immunohistochemistry

CD166/ALCAM Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Rat ALCAM / CD166 (rR ALCAM / CD166; Catalog#80221-R08H; O35112; Met1-Lys527). ALCAM / CD166 specific IgG was purified by Rat ALCAM / CD166 affinity chromatography.

CD166/ALCAM Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2000-1:10000
Notes  

CD166/ALCAM Antibody immunoprecipitation

CD166/ALCAM Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Rat CD166 / ALCAM (Catalog#80221-R08H; O35112; Met1-Lys527).

CD166/ALCAM Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

CD166/ALCAM Antibody ICC / IF

CD166/ALCAM Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166 / ALCAM (rh CD166 / ALCAM; Catalog#10045-H08H; NP_001618.2; Met1-Ala526). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD166/ALCAM Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD166/ALCAM antibody citations

Title
miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM
Year
2014
Author
Song, Q;Xu, Y;Yang, C;Chen, Z;Jia, C;Chen, J;Zhang, Y;Lai, P;Fan, X;Zhou, X;Lin, J;Li, M;Ma, W;Luo, S;Bai, X;
Journal
Cancer Res.
Title
Enzyme-linked antibody aptamer assays based colorimetric detection of soluble fraction of activated leukocyte cell adhesion molecule
Year
2016
Author
Her, J;Jo, H;Ban, C;
Journal
Sensors and Actuators B: Chemical
Title
Enzyme-linked antibody aptamer assays based colorimetric detection of soluble fraction of activated leukocyte cell adhesion molecule
Year
2016
Author
Her, J;Jo, H;Ban, C;
Journal
Sensors and Actuators B: Chemical