CD2 Antibody Guideline: Preparation, Dilution and Applications

CD2 Antibody Overview

There are 22 CD2 antibodies which are validated in multiple tissues with various applications, including FCM, ELISA, IHC-P, WB. There are 10 CD2 antibody for FCM, 10 CD2 antibody for ELISA, 2 CD2 antibody for IHC-P, 3 CD2 antibody for WB. Among all these CD2 antibodies, there are 8 anti-CD2 mouse monoclonal antibodies , 7 anti-CD2 rabbit monoclonal antibodies , 7 anti-CD2 rabbit polyclonal antibodies . All the CD2 anbodies are produced in house and all are in stock. CD2 antibody customerized service is available.

CD2 gene family antibody

CD2 Antibody ELISA

CD2 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD2 (rh CD2; Catalog#10982-H08H; NP_001758.2; Met1-Asp209). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.

CD2 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD2 Antibody western blot

CD2 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD2 (rM CD2; Catalog#50537-M08H; NP_038514.1; Met1-Ser203). CD2 specific IgG was purified by Mouse CD2 affinity chromatography.

CD2 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD2 Antibody flow cytometry

CD2 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD2 (rh CD2; Catalog#10982-H08H; NP_001758.2; Met1-Asp209). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD2 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD2 Antibody immunohistochemistry

CD2 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD2 (rM CD2; Catalog#50537-M08H; NP_038514.1; Met 1-Ser 203).

CD2 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes