CD200 Antibody Guideline: Preparation, Dilution and Applications

CD200 Antibody Overview

There are 12 CD200 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ELISA(Cap), IHC-P, WB. There are 5 CD200 antibody for ELISA, 5 CD200 antibody for FCM, 1 CD200 antibody for ELISA(Cap), 1 CD200 antibody for IHC-P, 2 CD200 antibody for WB. Among all these CD200 antibodies, there are 2 anti-CD200 mouse monoclonal antibodies , 5 anti-CD200 rabbit monoclonal antibodies , 5 anti-CD200 rabbit polyclonal antibodies . All the CD200 anbodies are produced in house and all are in stock. CD200 antibody customerized service is available.

CD200 Antibody ELISA

CD200 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD200 (rh CD200; Catalog#10886-H08H; NP_005935.4; Met 1-Gly 232). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD200 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD200 Antibody western blot

CD200 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD200 (rh CD200; Catalog#10886-H08H; NP_005935.4; Met1-Gly232). CD200 specific IgG was purified by Human CD200 affinity chromatography.

CD200 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD200 Antibody flow cytometry

CD200 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD200 (rh CD200; Catalog#10886-H08H; NP_005935.4; Met1-Gly232). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD200 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD200 Antibody immunohistochemistry

CD200 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD200 (rh CD200; Catalog#10886-H08H; NP_005935.4; Met1-Gly232).

CD200 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD200 antibody citations

Title
Evaluation of soluble CD200 levels in type 2 diabetic foot and nephropathic patients: association with disease activity
Year
2014
Author
Arik, HO;Yalcin, AD;Celik, B;Seyman, D;Tetik, G;Gursoy, B;Kose, S;Gumuslu, S;
Journal
Med. Sci. Monit.
Title
Evaluation of soluble CD200 levels in type 2 diabetic foot and nephropathic patients: association with disease activity
Year
2014
Author
Arik, HO;Yalcin, AD;Celik, B;Seyman, D;Tetik, G;Gursoy, B;Kose, S;Gumuslu, S;
Journal
Med. Sci. Monit.