CD3 zeta Antibody Guideline: Preparation, Dilution and Applications

CD3 zeta Antibody Overview

There are 2 CD3 zeta antibodies which are validated in multiple tissues with various applications, including ELISA, WB, IP. There are 2 CD3 zeta antibody for ELISA, 1 CD3 zeta antibody for WB, 1 CD3 zeta antibody for IP. Among all these CD3 zeta antibodies, there are 2 anti-CD3 zeta rabbit polyclonal antibodies . All the CD3 zeta anbodies are produced in house and all are in stock. CD3 zeta antibody customerized service is available.

CD3 zeta Antibody ELISA

CD3 zeta antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human CD247/CD3-ZETA (rh CD247/CD3-ZETA; Catalog#13142-H09E; P20963-1; Arg52-Arg164). CD247/CD3-ZETA specific IgG was purified by Human CD247/CD3-ZETA affinity chromatography.

CD3 zeta antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD3 zeta Antibody western blot

CD3 zeta Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD247 / CD3-ZETA (rh CD247 / CD3-ZETA; Catalog#13142-H09E; P20963-1; Arg52-Arg164). CD247 / CD3-ZETA specific IgG was purified by Human CD247 / CD3-ZETA affinity chromatography.

CD3 zeta Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD3 zeta Antibody immunoprecipitation

CD3 zeta Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD247 / CD3-ZETA (rh CD247 / CD3-ZETA; Catalog#13142-H09E; P20963-1; Arg52-Arg164). CD247 / CD3-ZETA specific IgG was purified by Human CD247 / CD3-ZETA affinity chromatography.

CD3 zeta Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-2 μL/mg of lysate
Notes