CD33 Antibody Guideline: Preparation, Dilution and Applications
CD33 Antibody Overview
There are 14 CD33 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB, IP. There are 8 CD33 antibody for ELISA, 8 CD33 antibody for FCM, 1 CD33 antibody for WB, 1 CD33 antibody for IP. Among all these CD33 antibodies, there are 3 anti-CD33 mouse monoclonal antibodies , 5 anti-CD33 rabbit monoclonal antibodies , 6 anti-CD33 rabbit polyclonal antibodies . All the CD33 anbodies are produced in house and all are in stock. CD33 antibody customerized service is available.
CD33 gene family antibody
| Approved symbol | Common name |
|---|---|
| TNFRSF8 | CD30/TNFRSF8 antibody |
| SIGLEC5 | SIGLEC5 antibody |
| CDH1 | E-Cadherin antibody |
| TNFSF11 | RANKL antibody |
| TNFRSF11A | RANK/TNFRSF11A antibody |
| IGF1R | IGF1R antibody |
| NT5E | CD73 antibody |
| CEACAM3 | CEACAM3 antibody |
| KIT | c-Kit antibody |
| TNFRSF9 | CD137 antibody |
| Approved symbol | Common name |
|---|---|
| CD93 | CD93 antibody |
| CD82 | CD82 antibody |
| JAM2 | JAM-2/JAM-B antibody |
| CD1B | CD1B antibody |
| CD8B | CD8 beta antibody |
| DPP4 | DPP4/CD26 antibody |
| CD180 | RP105/CD180 antibody |
| PDGFRB | PDGFRB antibody |
| PSG1 | PSG1 antibody |
| TNFSF14 | LIGHT antibody |
| Approved symbol | Common name |
|---|---|
| SIGLEC6 | SIGLEC6 antibody |
| SLAMF7 | SLAMF7/CD319 antibody |
| SIGLEC15 | SIGLEC15 antibody |
| TREM2 | TREM-2 antibody |
| AXL | AXL antibody |
| HEPACAM | HEPACAM antibody |
| PSG6 | PSG6 antibody |
| HHLA2 | HHLA2 antibody |
| TREML2 | TREML2 antibody |
| CD300LG | CLM-9 antibody |
| Approved symbol | Common name |
|---|---|
| NECTIN2 | Nectin-2 antibody |
| CD4 | CD4 antibody |
| CD300LF | CD300f/CD300LF antibody |
| PVR | CD155/PVR antibody |
| ICOSLG | ICOS ligand antibody |
| PTGFRN | PTGFRN antibody |
| HAVCR1 | TIM-1/KIM-1 antibody |
| ESAM | ESAM antibody |
| IGSF8 | IGSF8 antibody |
| GPA33 | A33 antibody |
| Approved symbol | Common name |
|---|---|
| CD22 | CD22 antibody |
| SIGLEC10 | SIGLEC10 antibody |
| SIGLEC5 | SIGLEC5 antibody |
| Approved symbol | Common name |
|---|---|
| MAG | MAG antibody |
| SIGLEC15 | SIGLEC15 antibody |
| SIGLEC6 | SIGLEC6 antibody |
CD33 Antibody ELISA
CD33 antibody ELISA preparation
Produced in rabbits immunized with purified, recombinant Human CD33 / Siglec-3 (rh CD33 / Siglec-3; Catalog#12238-H08H; AAH28152.1; Met1-His259). CD33 / Siglec-3 specific IgG was purified by Human CD33 / Siglec-3 affinity chromatography.
CD33 antibody ELISA validated
| Application | ELISA |
|---|---|
| Dilution | 1:5000-1:10000 |
| Notes |
CD33 Antibody western blot
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CD33 Antibody western blot Preparation
Produced in rabbits immunized with purified, recombinant Human CD33/Siglec-3 (rh CD33/Siglec-3; Catalog#12238-H08H; AAH28152.1; Met1-His259). CD33/Siglec-3 specific IgG was purified by Human CD33/Siglec-3 affinity chromatography.
CD33 Antibody western blot Validated
| Application | WB |
|---|---|
| Dilution | 1:500-1:1000 |
| Notes |
CD33 Antibody flow cytometry
CD33 Antibody flow cytometry Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD33 / Siglec-3 (rh CD33 / Siglec-3; Catalog#12238-H08H; AAH28152.1; Met1-His259). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD33 Antibody flow cytometry Validated
| Application | FC |
|---|---|
| Dilution | 1:25-1:100 |
| Notes |
CD33 Antibody immunoprecipitation
CD33 Antibody immunoprecipitation Preparation
Produced in rabbits immunized with purified, recombinant Human CD33/Siglec-3 (rh CD33/Siglec-3; Catalog#12238-H08H; AAH28152.1; Met1-His259). CD33/Siglec-3 specific IgG was purified by Human CD33/Siglec-3 affinity chromatography.
CD33 Antibody immunoprecipitation Validated
| Application | IP |
|---|---|
| Dilution | 0.2-1 μL/mg of lysate |
| Notes |
