CD36 Antibody Guideline: Preparation, Dilution and Applications

CD36 Antibody Overview

There are 12 CD36 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, WB, ICC/IF. There are 10 CD36 antibody for ELISA, 7 CD36 antibody for IHC-P, 2 CD36 antibody for WB, 1 CD36 antibody for ICC/IF. Among all these CD36 antibodies, there are 3 anti-CD36 mouse monoclonal antibodies , 4 anti-CD36 rabbit monoclonal antibodies , 5 anti-CD36 rabbit polyclonal antibodies . All the CD36 anbodies are produced in house and all are in stock. CD36 antibody customerized service is available.

CD36 Antibody ELISA

CD36 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD36 / SCARB3 (rh CD36 / SCARB3; Catalog#10752-H08H; NP_001001547.1; Gly30-Asn439). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD36 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD36 Antibody western blot

CD36 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD36 / SCARB3 (rh CD36 / SCARB3; Catalog#10752-H08H; NP_001001547.1; Gly30-Asn439).

CD36 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD36 Antibody immunohistochemistry

CD36 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD36 extracellular domain (rh CD36; Catalog#10752-H08H; NP_001001547.1; Gly30-Asn439). CD36 specific IgG was purified by human CD36 affinity chromatography.

CD36 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:5000-1:25000
Notes  

CD36 Antibody ICC / IF

CD36 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD36/SCARB3 (rM CD36/SCARB3; Catalog#50422-M08H; NP_001153030.1; Gly30-Lys439). CD36/SCARB3 specific IgG was purified by Mouse CD36/SCARB3 affinity chromatography.

CD36 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes  

CD36 antibody citations

Title
GCN2 deficiency ameliorates cardiac dysfunction in diabetic mice by reducing lipotoxicity and oxidative stress
Year
2018
Author
Feng, W;Lei, T;Wang, Y;Feng, R;Yuan, J;Shen, X;Wu, Y;Gao, J;Ding, W;Lu, Z;
Journal
Free Radic. Biol. Med.
Title
DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane
Year
2019
Author
Wang, J;Hao, JW;Wang, X;Guo, H;Sun, HH;Lai, XY;Liu, LY;Zhu, M;Wang, HY;Li, YF;Yu, LY;Xie, C;Wang, HR;Mo, W;Zhou, HM;Chen, S;Liang, G;Zhao, TJ;
Journal
Cell Rep