CD38 Antibody Guideline: Preparation, Dilution and Applications

CD38 Antibody Overview

There are 26 CD38 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, IHC-P, WB, IP, ELISA(Cap), ICC/IF. There are 14 CD38 antibody for ELISA, 8 CD38 antibody for FCM, 8 CD38 antibody for IHC-P, 3 CD38 antibody for WB, 3 CD38 antibody for IP, 1 CD38 antibody for ELISA(Cap), 1 CD38 antibody for ICC/IF. Among all these CD38 antibodies, there are 12 anti-CD38 mouse monoclonal antibodies , 7 anti-CD38 rabbit monoclonal antibodies , 7 anti-CD38 rabbit polyclonal antibodies . All the CD38 anbodies are produced in house and all are in stock. CD38 antibody customerized service is available.

CD38 Antibody ELISA

CD38 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD38 (rhCD38; Catalog#10818-H08H; NP_001766.2; Val 43-Ile 300). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD38 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD38 Antibody western blot

CD38 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD38 (rh CD38; Catalog#10818-H08H; NP_001766.2; Val 43-Ile 300).

CD38 Antibody western blot Validated

Application WB
Dilution 1:2000-1:10000
Notes  

CD38 Antibody flow cytometry

CD38 Antibody flow cytometry Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD38 (rM CD38; Catalog#50191-M08H; NP_031672.2; Leu 45-Thr 304). CD38 specific IgG was purified by mouse CD38 affinity chromatography .

CD38 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD38 Antibody immunohistochemistry

CD38 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD38 (rM CD38; Catalog#50191-M08H; NP_031672.2; Leu 45-Thr 304).

CD38 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:250-1:1000
Notes  

CD38 Antibody immunoprecipitation

CD38 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD38 (rhCD38; Catalog#10818-H08H; NP_001766.2; Val 43-Ile 300). CD38 specific IgG was purified by human CD38 affinity chromatography.

CD38 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

CD38 Antibody ICC / IF

CD38 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Rat CD38/ADPRC1 (rR CD38/ADPRC1; Catalog#80229-R08H; Q64244; Trp45-Val303). CD38/ADPRC1 specific IgG was purified by Rat CD38/ADPRC1 affinity chromatography.

CD38 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes  

CD38 antibody citations

Title
Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
Year
2019
Author
Gao, Z;Tong, C;Wang, Y;Chen, D;Wu, Z;Han, W;
Journal
J Genet Genomics
Title
Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
Year
2019
Author
Gao, Z;Tong, C;Wang, Y;Chen, D;Wu, Z;Han, W;
Journal
J Genet Genomics