CD3D Antibody Guideline: Preparation, Dilution and Applications

CD3D Antibody Overview

There are 10 CD3D antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA(Cap), ELISA, ELISA(Det), IP. There are 6 CD3D antibody for WB, 1 CD3D antibody for IHC-P, 1 CD3D antibody for ELISA(Cap), 5 CD3D antibody for ELISA, 1 CD3D antibody for ELISA(Det), 5 CD3D antibody for IP. Among all these CD3D antibodies, there are 3 anti-CD3D rabbit polyclonal antibodies , 5 anti-CD3D mouse monoclonal antibodies , 2 anti-CD3D rabbit monoclonal antibodies . All the CD3D anbodies are produced in house and all are in stock. CD3D antibody customerized service is available.

CD3D Antibody ELISA

CD3D Antibody western blot

CD3D Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse CD3d / CD3 delta, and purified by antigen affinity chromatography.

CD3D Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD3D Antibody immunohistochemistry

CD3D Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3d / CD3 delta (rh CD3d / CD3 delta; Catalog#10981-H08H; NP_000723.1; Met 1-Ala 105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD3D Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD3D Antibody immunoprecipitation

CD3D Antibody immunoprecipitation Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3D (rh CD3D; Catalog#10981-H08H; NP_000723.1; Met 1-Ala 105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD3D Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes