CD4 Antibody Guideline: Preparation, Dilution and Applications
CD4 Antibody Overview
There are 44 CD4 antibodies which are validated in multiple tissues with various applications, including FCM, ELISA, WB, IHC-P, ICC/IF, IP, ELISA(Det), ELISA(Cap). There are 22 CD4 antibody for FCM, 15 CD4 antibody for ELISA, 4 CD4 antibody for WB, 7 CD4 antibody for IHC-P, 1 CD4 antibody for ICC/IF, 1 CD4 antibody for IP, 1 CD4 antibody for ELISA(Det), 1 CD4 antibody for ELISA(Cap). Among all these CD4 antibodies, there are 22 anti-CD4 mouse monoclonal antibodies , 15 anti-CD4 rabbit monoclonal antibodies , 7 anti-CD4 rabbit polyclonal antibodies . All the CD4 anbodies are produced in house and all are in stock. CD4 antibody customerized service is available.
CD4 gene family antibody
| Approved symbol | Common name |
|---|---|
| TNFRSF21 | DR6 antibody |
| CD244 | 2B4/CD244 antibody |
| CEACAM3 | CEACAM3 antibody |
| CD8A | CD8 alpha antibody |
| LAIR1 | LAIR1 antibody |
| CD300E | CD300E antibody |
| KIR2DL4 | KIR2DL4 antibody |
| GYPA | Glycophorin A antibody |
| CD160 | CD160 antibody |
| CDH2 | N Cadherin antibody |
| Approved symbol | Common name |
|---|---|
| TLR2 | TLR2 antibody |
| CD3D | CD3D antibody |
| ICOSLG | ICOS ligand antibody |
| TNFRSF14 | HVEM antibody |
| IL2RG | IL2RG antibody |
| F11R | JAM-A/Junctional Adhesion Molecule A antibody |
| L1CAM | L1CAM antibody |
| THBD | Thrombomodulin antibody |
| HAVCR1 | TIM-1/KIM-1 antibody |
| MELTF | MFI2 antibody |
| Approved symbol | Common name |
|---|---|
| CD8B | CD8 beta antibody |
| TREM2 | TREM-2 antibody |
| CTLA4 | CTLA-4 antibody |
| TIGIT | TIGIT antibody |
| SIGLEC15 | SIGLEC15 antibody |
| CEACAM5 | CEACAM5 antibody |
| CD80 | B7-1 antibody |
| VTCN1 | B7-H4 antibody |
| HHLA2 | HHLA2 antibody |
| HAPLN1 | HAPLN1 antibody |
| Approved symbol | Common name |
|---|---|
| HAVCR2 | TIM-3 antibody |
| SIGLEC10 | SIGLEC10 antibody |
| CD300C | CD300C antibody |
| CXADR | CAR/CXADR antibody |
| ICOSLG | ICOS ligand antibody |
| BTN3A1 | BTN3A1 antibody |
| TIMD4 | Tim4/TIMD4 antibody |
| PILRA | PILRA antibody |
| PSG6 | PSG6 antibody |
| CADM4 | CADM4 antibody |
| Approved symbol | Common name |
|---|---|
| ICOSLG | ICOS ligand antibody |
| SIGLEC10 | SIGLEC10 antibody |
| KIRREL3 | KIRREL3 antibody |
| NECTIN4 | NECTIN4/Nectin 4 antibody |
| KIRREL2 | KIRREL2 antibody |
| CRTAM | CRTAM antibody |
| CD22 | CD22 antibody |
| AGER | RAGE antibody |
| CD274 | PD-L1 antibody |
| MAG | MAG antibody |
| Approved symbol | Common name |
|---|---|
| BTN3A1 | BTN3A1 antibody |
| CD200R1 | CD200R antibody |
| VCAM1 | VCAM1 antibody |
| CD80 | B7-1 antibody |
| BCAM | BCAM antibody |
| CD276 | B7-H3 antibody |
| TMEM25 | TMEM25 antibody |
| KIRREL1 | KIRREL antibody |
| CADM1 | CADM1 antibody |
| ALCAM | CD166/ALCAM antibody |
CD4 Antibody ELISA
CD4 antibody ELISA preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met 1-Trp 390). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD4 antibody ELISA validated
| Application | ELISA |
|---|---|
| Dilution | 1:1000-1:2000 |
| Notes |
CD4 Antibody western blot
CD4 Antibody western blot Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met1-Trp390). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD4 Antibody western blot Validated
| Application | WB |
|---|---|
| Dilution | 1:500-1:1000 |
| Notes |
CD4 Antibody flow cytometry
CD4 Antibody flow cytometry Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD4 (rF CD4; Catalog#60003-F08H; ABS50090.1; Met 1-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD4 Antibody flow cytometry Validated
| Application | FC |
|---|---|
| Dilution | 1:25-1:100 |
| Notes |
CD4 Antibody immunohistochemistry
CD4 Antibody immunoprecipitation
CD4 Antibody immunoprecipitation Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD4 (rM CD4; Catalog#50134-M08H; NP_038516.1; Met1-Thr394). CD4 specific IgG was purified by Mouse CD4 affinity chromatography.
CD4 Antibody immunoprecipitation Validated
| Application | IP |
|---|---|
| Dilution | 0.1-0.5 μL/mg of lysate |
| Notes |
CD4 antibody citations
Title
An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
Year
2014
Author
M Bonsignori, K Wiehe, SK Grimm…
Journal
The Journal of Clinical Investigation
Title
An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
Year
2014
Author
M Bonsignori, K Wiehe, SK Grimm…
Journal
The Journal of Clinical Investigation
Title
Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
Year
2017
Author
Sun, X;Belser, JA;Pulit-Penaloza, JA;Creager, HM;Guo, Z;Jefferson, SN;Liu, F;York, IA;Stevens, J;Maines, TR;Jernigan, DB;Katz, JM;Levine, MZ;Tumpey, TM;
Journal
Virology
Title
The immune correlates of protection for an avian influenza H5N1 vaccine in the ferret model using oil-in-water adjuvants
Year
2017
Author
Wong, SS;Duan, S;DeBeauchamp, J;Zanin, M;Kercher, L;Sonnberg, S;Fabrizio, T;Jeevan, T;Crumpton, JC;Oshansky, C;Sun, Y;Tang, L;Thomas, P;Webby, R;
Journal
Sci Rep
Title
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model
Year
2018
Author
Reber, AJ;Music, N;Kim, JH;Gansebom, S;Chen, J;York, I;
Journal
Sci Rep
Title
TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis
Year
2018
Author
Tauriello, DVF;Palomo-Ponce, S;Stork, D;Berenguer-Llergo, A;Badia-Ramentol, J;Iglesias, M;Sevillano, M;Ibiza, S;Cañellas, A;Hernando-Momblona, X;Byrom, D;Matarin, JA;Calon, A;Rivas, EI;Nebreda, AR;Riera, A;Attolini, CS;Batlle, E;
Journal
Nature
Title
The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms
Year
2018
Author
Balbinot, C;Armant, O;Elarouci, N;Marisa, L;Martin, E;De Clara, E;Onea, A;Deschamps, J;Beck, F;Freund, JN;Duluc, I;
Journal
J. Exp. Med.
Title
Comparative Pathogenesis of Influenza in Ferrets
Year
2020
Author
Ryan, KA;
Journal
Thesis
Title
Gold nanoparticle-streptavidin conjugates for rapid and efficient screening of aptamer function in lateral flow sensors using novel CD4-binding aptamers identified through Crossover-SELEX
Year
2020
Author
Fellows, T;Ho, L;Flanagan, S;Fogel, R;Ojo, D;Limson, J;
Journal
Analyst
Title
The targeted delivery of interleukin-12 to the carcinoembryonic antigen increases the intratumoral density of NK and CD8+ T cell in an immunocompetent mouse model of colorectal cancer
Year
2020
Author
Puca, E;Schmitt-Koopmann, C;Furter, M;Murer, P;Probst, P;Dihr, M;Bajic, D;Neri, D;
Journal
J Gastrointest Oncol
