CD40 Antibody Guideline: Preparation, Dilution and Applications

CD40 Antibody Overview

There are 28 CD40 antibodies which are validated in multiple tissues with various applications, including WB, IP, IHC-P, ICC/IF, FCM, ELISA, ELISA(Cap), ELISA(Det). There are 3 CD40 antibody for WB, 1 CD40 antibody for IP, 2 CD40 antibody for IHC-P, 3 CD40 antibody for ICC/IF, 12 CD40 antibody for FCM, 12 CD40 antibody for ELISA, 1 CD40 antibody for ELISA(Cap), 1 CD40 antibody for ELISA(Det). Among all these CD40 antibodies, there are 11 anti-CD40 rabbit polyclonal antibodies , 15 anti-CD40 mouse monoclonal antibodies , 2 anti-CD40 rabbit monoclonal antibodies . All the CD40 anbodies are produced in house and all are in stock. CD40 antibody customerized service is available.

CD40 Antibody ELISA

CD40 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD40 extracellular domain (rh CD40; Catalog#10774-H08H; NP_001241.1; Met 1-Arg 193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD40 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD40 Antibody western blot

CD40 Antibody western blot

CD40 Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CD40/TNFRSF5, and purified by antigen affinity chromatography.

CD40 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD40 Antibody flow cytometry

CD40 Antibody flow cytometry

CD40 Antibody immunohistochemistry

CD40 Antibody immunohistochemistry

CD40 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD40/TNFRSF5 (rh CD40/TNFRSF5; Catalog#10774-H08H; NP_001241.1; Met1-Arg193). CD40/TNFRSF5 specific IgG was purified by Human CD40/TNFRSF5 affinity chromatography.

CD40 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:1000-1:4000
Notes  

CD40 Antibody immunoprecipitation

CD40 Antibody immunoprecipitation

CD40 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CD40 / TNFRSF5, and purified by antigen affinity chromatography.

CD40 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

CD40 Antibody ICC / IF

CD40 Antibody ICC / IF

CD40 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD40 / TNFRSF5 (rh CD40 / TNFRSF5; Catalog#10774-H08H; NP_001241.1; Met1-Arg193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD40 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD40 antibody citations

Title
Dendritic-cell-based immunotherapy evokes potent anti-tumor immune responses in CD105+ human renal cancer stem cells
Year
2017
Author
Zhang, XF;Weng, DS;Pan, K;Zhou, ZQ;Pan, QZ;Zhao, JJ;Tang, Y;Jiang, SS;Chen, CL;Li, YQ;Zhang, HX;Chang, AE;Wicha, MS;Zeng, YX;Li, Q;Xia, JC;
Journal
Mol. Carcinog.