CD44 Antibody Guideline: Preparation, Dilution and Applications

CD44 Antibody Overview

There are 12 CD44 antibodies which are validated in multiple tissues with various applications, including IHC-P, WB, ELISA, ELISA(Cap), IP, FCM, ICC/IF, ELISA(Det). There are 3 CD44 antibody for IHC-P, 4 CD44 antibody for WB, 5 CD44 antibody for ELISA, 1 CD44 antibody for ELISA(Cap), 3 CD44 antibody for IP, 5 CD44 antibody for FCM, 3 CD44 antibody for ICC/IF, 1 CD44 antibody for ELISA(Det). Among all these CD44 antibodies, there are 3 anti-CD44 rabbit polyclonal antibodies , 7 anti-CD44 mouse monoclonal antibodies , 2 anti-CD44 rabbit monoclonal antibodies . All the CD44 anbodies are produced in house and all are in stock. CD44 antibody customerized service is available.

CD44 Antibody ELISA

CD44 antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220).

CD44 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD44 Antibody western blot

CD44 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). CD44 specific IgG was purified by Human CD44 affinity chromatography.

CD44 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD44 Antibody flow cytometry

CD44 Antibody immunohistochemistry

CD44 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse CD44, and purified by antigen affinity chromatography.

CD44 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CD44 Antibody immunoprecipitation

CD44 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met1-Pro220). CD44 specific IgG was purified by Human CD44 affinity chromatography.

CD44 Antibody immunoprecipitation Validated

Application IP
Dilution IP:0.5-2 μL/mg of lysate
Notes  

CD44 Antibody ICC / IF

CD44 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met1-Pro220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD44 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD44 antibody citations

Title
Establishment and Characterization of a Highly Metastatic Ovarian Cancer Cell Line
Year
2018
Author
Ruibin, J;Guoping, C;Zhiguo, Z;Maowei, N;Danying, W;Jianguo, F;Linhui, G;
Journal
Biomed Res Int
Title
Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment
Year
2018
Author
Fu, JJ;Zhou, Y;Shi, XX;Kang, YJ;Lu, ZS;Li, Y;Li, CM;Yu, L;
Journal
Colloids Surf B Biointerfaces