CD47 Antibody Guideline: Preparation, Dilution and Applications

CD47 Antibody Overview

There are 15 CD47 antibodies which are validated in multiple tissues with various applications, including IHC-P, WB, ELISA, FCM, ICC/IF. There are 2 CD47 antibody for IHC-P, 2 CD47 antibody for WB, 10 CD47 antibody for ELISA, 3 CD47 antibody for FCM, 1 CD47 antibody for ICC/IF. Among all these CD47 antibodies, there are 8 anti-CD47 rabbit polyclonal antibodies , 5 anti-CD47 mouse monoclonal antibodies , 2 anti-CD47 rabbit monoclonal antibodies . All the CD47 anbodies are produced in house and all are in stock. CD47 antibody customerized service is available.

CD47 gene family antibody

CD47 Antibody ELISA

CD47 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD47 (rh CD47; Catalog#12283-HCCH; NP_942088.1; Met1-Pro139). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD47 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD47 Antibody western blot

CD47 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Rat CD47 (Catalog#80305-R08H; P97829-1; Met1-Lys140). CD47 specific IgG was purified by Rat CD47 affinity chromatography.

CD47 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD47 Antibody flow cytometry

CD47 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD47 (rh CD47; Catalog#12283-HCCH; NP_942088.1; Met1-Pro139). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD47 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD47 Antibody immunohistochemistry

CD47 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse CD47, and purified by antigen affinity chromatography.

CD47 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2500-1:10000
Notes  

CD47 Antibody ICC / IF

CD47 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human CD47 (rh CD47; Catalog#12283-H08H; NP_942088.1; Met1-Pro139). CD47 specific IgG was purified by Human CD47 affinity chromatography.

CD47 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes