CD5 Antibody Guideline: Preparation, Dilution and Applications

CD5 Antibody Overview

There are 41 CD5 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB, IHC-P, IP, ICC/IF, ELISA(Cap). There are 13 CD5 antibody for ELISA, 26 CD5 antibody for FCM, 5 CD5 antibody for WB, 4 CD5 antibody for IHC-P, 1 CD5 antibody for IP, 3 CD5 antibody for ICC/IF, 1 CD5 antibody for ELISA(Cap). Among all these CD5 antibodies, there are 8 anti-CD5 mouse monoclonal antibodies , 25 anti-CD5 rabbit monoclonal antibodies , 8 anti-CD5 rabbit polyclonal antibodies . All the CD5 anbodies are produced in house and all are in stock. CD5 antibody customerized service is available.

CD5 Antibody ELISA

CD5 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD5 (rh CD5; Catalog#11027-H08H; NP_055022.2; Met 1-Pro 372). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD5 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD5 Antibody western blot

CD5 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD5 / Cluster of Differentiation 5 (rh CD5 / Cluster of Differentiation 5; Catalog#11027-H08H; NP_055022.2; Met1-Pro372).

CD5 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD5 Antibody flow cytometry

CD5 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD5 (rh CD5; Catalog#11027-H08H; NP_055022.2; Met 1-Pro 372).

CD5 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD5 Antibody immunohistochemistry

CD5 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD5 / Cluster of Differentiation 5 (rh CD5 / Cluster of Differentiation 5; Catalog#11027-H08H; NP_055022.2; Met1-Pro372). CD5 / Cluster of Differentiation 5 specific IgG was purified by Human CD5 / Cluster of Differentiation 5 affinity chromatography.

CD5 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:1000-1:5000
Notes  

CD5 Antibody immunoprecipitation

CD5 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD5 (rh CD5; Catalog#11027-H08H; NP_055022.2; Met 1-Pro 372). CD5 specific IgG was purified by Human CD5 affinity chromatography.

CD5 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

CD5 Antibody ICC / IF

CD5 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD5 / Cluster of Differentiation 5 (rM CD5 / Cluster of Differentiation 5; Catalog#50403-M08H; NP_031676.3; Met1-Pro371).

CD5 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes