CD55/DAF Antibody Guideline: Preparation, Dilution and Applications

CD55/DAF Antibody Overview

There are 22 CD55/DAF antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Det), FCM, ELISA(Cap), ICC/IF, WB, IHC-P, IP. There are 8 CD55/DAF antibody for ELISA, 1 CD55/DAF antibody for ELISA(Det), 15 CD55/DAF antibody for FCM, 2 CD55/DAF antibody for ELISA(Cap), 2 CD55/DAF antibody for ICC/IF, 1 CD55/DAF antibody for WB, 1 CD55/DAF antibody for IHC-P, 1 CD55/DAF antibody for IP. Among all these CD55/DAF antibodies, there are 7 anti-CD55/DAF mouse monoclonal antibodies , 11 anti-CD55/DAF rabbit monoclonal antibodies , 4 anti-CD55/DAF rabbit polyclonal antibodies . All the CD55/DAF anbodies are produced in house and all are in stock. CD55/DAF antibody customerized service is available.

CD55/DAF Antibody ELISA

CD55/DAF antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD55 / DAF (rh CD55; Catalog#10101-H08H; NP_000565.1; Met 1-Ser 353). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD55/DAF antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD55/DAF Antibody western blot

CD55/DAF Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD55 / DAF (rh CD55 / DAF; Catalog#10101-H08H; NP_000565.1; Met1-Ser353). CD55 / DAF specific IgG was purified by Human CD55 / DAF affinity chromatography.

CD55/DAF Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD55/DAF Antibody flow cytometry

CD55/DAF Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD55 / DAF (rh CD55 / DAF; Catalog#10101-H08H; NP_000565.1; Met1-Ser353). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD55/DAF Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD55/DAF Antibody immunohistochemistry

CD55/DAF Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CD55 / DAF (rh CD55 / DAF; Catalog#10101-H08H; NP_000565.1; Met1-Ser353). CD55 / DAF specific IgG was purified by Human CD55 / DAF affinity chromatography.

CD55/DAF Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CD55/DAF Antibody immunoprecipitation

CD55/DAF Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD55 / DAF (rh CD55 / DAF; Catalog#10101-H08H; NP_000565.1; Met1-Ser353). CD55 / DAF specific IgG was purified by Human CD55 / DAF affinity chromatography.

CD55/DAF Antibody immunoprecipitation Validated

Application IP
Dilution 1-5 μL/mg of lysate
Notes  

CD55/DAF Antibody ICC / IF

CD55/DAF Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55 / DAF (rh CD55; Catalog#10101-H08H; NP_000565.1; Met 1-Ser 353).

CD55/DAF Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD55/DAF antibody citations

Title
Complement regulatory proteins (CD46, 55 and 59) expressed on Schwann cells: Immune targets in demyelinating neuropathies?
Year
2014
Author
Miyaji, K;Paul, F;Shahrizaila, N;Umapathi, T;Yuki, N;
Journal
J. Neuroimmunol.