CD58 Antibody Guideline: Preparation, Dilution and Applications

CD58 Antibody Overview

There are 16 CD58 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB, IP, IHC-P, ICC/IF. There are 5 CD58 antibody for ELISA, 8 CD58 antibody for FCM, 4 CD58 antibody for WB, 1 CD58 antibody for IP, 1 CD58 antibody for IHC-P, 1 CD58 antibody for ICC/IF. Among all these CD58 antibodies, there are 5 anti-CD58 mouse monoclonal antibodies , 8 anti-CD58 rabbit monoclonal antibodies , 3 anti-CD58 rabbit polyclonal antibodies . All the CD58 anbodies are produced in house and all are in stock. CD58 antibody customerized service is available.

CD58 Antibody ELISA

CD58 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD58 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD58 Antibody western blot

CD58 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215).

CD58 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD58 Antibody flow cytometry

CD58 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215).

CD58 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD58 Antibody immunohistochemistry

CD58 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215).

CD58 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

CD58 Antibody immunoprecipitation

CD58 Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215).

CD58 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

CD58 Antibody ICC / IF

CD58 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215). LFA-3 / CD58 specific IgG was purified by Human LFA-3 / CD58 affinity chromatography.

CD58 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:1500-1:50000
Notes