CD97 Antibody Guideline: Preparation, Dilution and Applications

CD97 Antibody Overview

There are 15 CD97 antibodies which are validated in multiple tissues with various applications, including WB, IP, FCM, ICC/IF, ELISA, ELISA(Det), ELISA(Cap). There are 3 CD97 antibody for WB, 3 CD97 antibody for IP, 8 CD97 antibody for FCM, 4 CD97 antibody for ICC/IF, 7 CD97 antibody for ELISA, 1 CD97 antibody for ELISA(Det), 1 CD97 antibody for ELISA(Cap). Among all these CD97 antibodies, there are 4 anti-CD97 rabbit polyclonal antibodies , 7 anti-CD97 mouse monoclonal antibodies , 4 anti-CD97 rabbit monoclonal antibodies . All the CD97 anbodies are produced in house and all are in stock. CD97 antibody customerized service is available.

CD97 Antibody ELISA

CD97 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD97 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD97 Antibody western blot

CD97 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met1-Gln398). CD97 specific IgG was purified by Human CD97 affinity chromatography.

CD97 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD97 Antibody flow cytometry

CD97 Antibody immunoprecipitation

CD97 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398). CD97 specific IgG was purified by human CD97 affinity chromatography.

CD97 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

CD97 Antibody ICC / IF

CD97 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398).

CD97 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes