CDK2 Protein Overview: Sequence, Structure, Function and Protein Interaction

CDK2 Protein Overview

Ninomiya-Tsuji et al. (1991) cloned 2 different cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponded to a gene encoding human p34(CDC2) kinase (116940), and the other to a gene that had not been characterized previously, CDK2 (cell division kinase-2). The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The human CDC2 and CDK2 genes were both able to complement the inviability of a null allele of S. cerevisiae, CDC28. However, CDK2 was unable to complement cdc2 mutants in fission yeast Schizosaccharomyces pombe under the condition where the human CDC2 gene could complement them. CDK2 mRNA appeared late in G1 or in early S phase, slightly before CDC2 mRNA, after growth stimulation in the normal human fibroblast cells. Thus, 2 different CDC2-like kinases appear to regulate the human cell cycle at different stages.

CDK2 protein family

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

CDK2 protein name

Recommended name
Cyclin-dependent kinase 2
Alternative name
Cell division protein kinase 2 p33 protein kinase

CDK2 Gene family protein

CDK2 Protein Sequence

Species Human CDK2 protein
Length 298
Mass (Da) 33930
Sequence Human CDK2 protein sequence
Species Mouse CDK2 protein
Length 346
Mass (Da) 38978
Sequence Mouse CDK2 protein sequence
Species Rat CDK2 protein
Length 298
Mass (Da) 33887
Sequence Rat CDK2 protein sequence

CDK2 Protein Molecular Weight & PI

Cyclin-dependent kinase 2 (EC 2.7.11.22) (Cell division protein kinase 2) (p33 protein kinase) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-298 Cyclin-dependent kinase 2.

Molecular weight (Da)

33929.53

Theoretical pI

8.80

CDK2 Protein Structure

Crystal structure of human Cdk2-Spy1 complex
Deposited
2017-02-06   Released:  2017-07-05
Deposition Author(s)
McGrath, D.A., Tripathi, S.M., Rubin, S.M.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.2010 Å
R-Value Free
0.261
R-Value Work
0.205
5UQ1 From PDB

Human CDK2 protein Secondary structure

CDK2 Protein Interaction

Recombinant CDK2 Protein Feature

CDK2 Protein, Human, Recombinant (His Tag)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
No Kinase Activity

Recombinant CDK2 protein citations

Title
Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2
Year
2020
Author
Liu, YC;Feng, N;Li, WW;Tu, PF;Chen, JP;Han, JY;Zeng, KW;
Journal
Molecules

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