CNTF Protein Overview: Sequence, Structure, Function and Protein Interaction

CNTF Protein Overview

By analysis of human-hamster somatic cell hybrids, Lam et al. (1991) mapped the CNTF gene to chromosome 11. Using a rodent/human somatic cell DNA mapping panel and fluorescence in situ hybridization, Lev et al. (1993) localized the CNTF gene to the proximal region of 11q. In addition, they identified a polymorphic tandem CA/GT dinucleotide repeat associated with the human CNTF gene. To sublocalize the CNTF gene on chromosome 11, Giovannini et al. (1993) isolated cosmid clones containing the gene by use of a chromosome 11-specific library. Using these clones in fluorescence in situ hybridization, they found that the gene maps at an FLpter of 0.46, corresponding to a cytogenetic band position of 11q12.2, according to the method of Lichter et al. (1990). Yokoji et al. (1995) isolated a full-length cDNA for human ciliary neurotrophic factor from a sciatic nerve cDNA library, determined its structure, and localized it to chromosome 11q12 by fluorescence in situ hybridization. Kaupmann et al. (1991) demonstrated that the mouse Cntf gene is on mouse chromosome 19 and that its expression is unaffected in the mouse neurologic mutant wobbler (see 614633), a form of spinal muscular atrophy.

CNTF protein family

Belongs to the CNTF family.

CNTF protein name

Recommended name
Ciliary neurotrophic factor
Short name
CNTF
Aliases
HCNTF

CNTF Protein Sequence

Species Human CNTF protein
Length 200
Mass (Da) 22931
Sequence Human CNTF protein sequence
Species Mouse CNTF protein
Length 198
Mass (Da) 22587
Sequence Mouse CNTF protein sequence
Species Rat CNTF protein
Length 200
Mass (Da) 22854
Sequence Rat CNTF protein sequence

CNTF Protein Molecular Weight & PI

Ciliary neurotrophic factor (CNTF) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-200 Ciliary neurotrophic factor.

Molecular weight (Da)

22931.13

Theoretical pI

6.35

CNTF Protein Structure

CILIARY NEUROTROPHIC FACTOR
Deposited
1996-06-06   Released:  1997-03-26
Deposition Author(s)
Mcdonald, N.Q., Panayotatos, N., Hendrickson, W.A.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.4000 Å
R-Value Free
0.247
R-Value Work
0.199
1CNT From PDB

Human CNTF protein Secondary structure

CNTF Protein Interaction

Recombinant CNTF Protein Feature

CNTF Protein, Mouse, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF (cat:51181-M07E) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (Cat:80019-R08B). The EC50 of biotinylated rat CNTFR-His (Cat:80019-R08B) is 5-12 ng/ml.

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