Cyclin E Antibody Guideline: Preparation, Dilution and Applications

Cyclin E Antibody Overview

There are 5 Cyclin E antibodies which are validated in multiple tissues with various applications, including ICC/IF, FCM, IHC-P, ELISA, WB. There are 2 Cyclin E antibody for ICC/IF, 1 Cyclin E antibody for FCM, 2 Cyclin E antibody for IHC-P, 2 Cyclin E antibody for ELISA, 1 Cyclin E antibody for WB. Among all these Cyclin E antibodies, there are 3 anti-Cyclin E mouse monoclonal antibodies , 2 anti-Cyclin E rabbit polyclonal antibodies . All the Cyclin E anbodies are produced in house and all are in stock. Cyclin E antibody customerized service is available.

Cyclin E gene family antibody

Approved symbol Common name
CCNA1 Cyclin A1 antibody

Cyclin E Antibody ELISA

Cyclin E antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human CCNE1 (rh CCNE1 , Catalog#10902-H07B; NP_001229.1; Met 1-Ala 410). Total IgG was purified by Protein A affinity chromatography .

Cyclin E antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Cyclin E Antibody western blot

Cyclin E Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CCNE1 (rh CCNE1 , Catalog#10902-H07B; NP_001229.1; Met 1-Ala 410). CCNE1 specific IgG was purified by human CCNE1 affinity chromatography .

Cyclin E Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Cyclin E Antibody flow cytometry

Cyclin E Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cyclin E/CCNE1 (rh Cyclin E/CCNE1; Catalog#10902-H07B; NP_001229.1; Met1-Ala410). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Cyclin E Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

Cyclin E Antibody immunohistochemistry

Cyclin E Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCNE1 / Cyclin-E1 (rh CCNE1 / Cyclin-E1; Catalog#10902-H07B; NP_001229.1; Met1-Ala410). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Cyclin E Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

Cyclin E Antibody ICC / IF

Cyclin E Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCNE1 / Cyclin-E1 (rh CCNE1 / Cyclin-E1; Catalog#10902-H07B; NP_001229.1; Met1-Ala410). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Cyclin E Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

Cyclin E antibody citations

Title
Impacts of CyhospitalclinE downstream vimentin on proliferation, invasion and apoptosis of hepatoma HepG2 cell
Year
2018
Author
Hu, G;Jia, F;Gao, N;Han, Y;
Journal
International Journal of Clinical and Experimental Medicine
Title
NVP-BEZ235 inhibits thyroid cancer growth by p53-dependent/independent p21 upregulation
Year
2020
Author
Ruan, B;Liu, W;Chen, P;Cui, R;Li, Y;Ji, M;Hou, P;
Journal
Int J Biol Sci