FCRN/FCGRT Protein Overview: Sequence, Structure, Function and Protein Interaction

FCRN/FCGRT Protein Overview

The intestinal epithelium of neonatal mice and rats expresses an Fc receptor (FcRn) that mediates the selective uptake of immunoglobulin G (IgG) in mothers' milk, thereby helping newborn animals to acquire passive immunity. Kandil et al. (1996) noted that the IgG in the milk is bound to FcRn at the apical surface of the intestinal epithelium, and the resultant FcRn-IgG complexes are transcytosed across the epithelium. At the basolateral surface of intestinal epithelial cells, IgG is released from FcRn into blood or tissue fluids. FcRn is structurally similar to the major histocompatibility complex class I molecule, which presents antigenic peptides to T cells. Like the MHC class I molecule, FcRn is made up of a heavy chain (approximately 48 kD) and beta-2-microglobulin (109700; approximately 12 kD). Its heavy chain shows approximately 35% amino acid sequence identity to that of a typical MHC class I molecule. Furthermore, the genomic organizations of the MHC class I and mouse FcRn heavy chain genes are similar in that the signal peptide, the 3 extracellular domains, the transmembrane region, and the cytoplasmic region are all encoded by separate exons. Story et al. (1994) cloned a cDNA encoding the human gene and symbolized it FcRn. Kandil et al. (1996) isolated human genomic clones for the gene (symbolized FCGRT by them) using 2 murine Fcgrt probes. The exonic sequences of the genomic clones are identical to the cDNA sequence described by Story et al. (1994).

FCRN/FCGRT protein family

Belongs to the immunoglobulin superfamily.

FCRN/FCGRT protein name

Recommended name
IgG receptor FcRn large subunit p51
Short name
FcRn
Aliases
alpha-chain, FCRN, heavy chain of the major histocompatibility complex class I-like Fc receptor, transmembrane alpha chain of the neonatal receptor
Alternative name
IgG Fc fragment receptor transporter alpha chain Neonatal Fc receptor

FCRN/FCGRT Protein Sequence

Species Human FCRN/FCGRT protein
Length 365
Mass (Da) 39743
Sequence Human FCRN/FCGRT protein sequence
Species Mouse FCRN/FCGRT protein
Length 365
Mass (Da) 40093
Sequence Mouse FCRN/FCGRT protein sequence
Species Rat FCRN/FCGRT protein
Length 366
Mass (Da) 40169
Sequence Rat FCRN/FCGRT protein sequence

FCRN/FCGRT Protein Molecular Weight & PI

IgG receptor FcRn large subunit p51 precursor (FcRn) (IgG Fc fragment receptor transporter alpha chain) (Neonatal Fc receptor) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 24-365 IgG receptor FcRn large subunit p51.

Molecular weight (Da)

37328.37

Theoretical pI

5.94

FCRN/FCGRT Protein Structure

Crystal structure of human FcRn with a monomeric peptide inhibitor
Deposited
2010-03-04   Released:  2010-06-16
Deposition Author(s)
Mezo, A.R., Sridhar, V., Badger, J., Sakorafas, P., Nienaber, V.
Organism(s)
Homo sapiens
Expression System
Cricetinae
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.6000 Å
R-Value Free
0.325
R-Value Work
0.261
3M17 From PDB

Human FCRN/FCGRT protein Secondary structure

FCRN/FCGRT Protein Interaction

Recombinant FCRN/FCGRT Protein Feature

FCRN/FCGRT Protein, Human, Recombinant (ECD, His & AVI Tag)

High Purity
> 95 % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.

Recombinant FCRN/FCGRT protein citations

Title
Fc containing polypeptides having increased anti-inflammatory properties and increased fcrn binding
Year
2012
Author
D Zha
Journal
US Patent App. 14/357,779
Title
Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury
Year
2017
Author
Pyzik, M;Rath, T;Kuo, TT;Win, S;Baker, K;Hubbard, JJ;Grenha, R;Gandhi, A;Krämer, TD;Mezo, AR;Taylor, ZS;McDonnell, K;Nienaber, V;Andersen, JT;Mizoguchi, A;Blumberg, L;Purohit, S;Jones, SD;Christianson, G;Lencer, WI;Sandlie, I;Kaplowitz, N;Roopenian, DC;Blumberg, RS;
Journal
Proc. Natl. Acad. Sci. U.S.A
Title
Increased half-life and enhanced potency of Fc-modified human PCSK9 monoclonal antibodies in primates
Year
2017
Author
Shen, Y;Li, H;Zhao, L;Li, G;Chen, B;Guo, Q;Gao, B;Wu, J;Yang, T;Jin, L;Su, Y;
Journal
PLoS ONE
Title
Modulation of protein A binding allows single-step purification of mouse bispecific antibodies that retain FcRn binding
Year
2017
Author
Zwolak, A;Armstrong, AA;Tam, SH;Pardinas, JR;Goulet, DR;Zheng, S;Brosnan, K;Emmell, E;Luo, J;Gilliland, GL;Chiu, ML;
Journal
MAbs
Title
Rapid screening of IgG quality attributes: effects on Fc receptor binding
Year
2017
Author
Geuijen, KP;Oppers-Tiemissen, C;Egging, DF;Simons, PJ;Boon, L;Schasfoort, RB;Eppink, MH;
Journal
FEBS Open Bio
Title
Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding
Year
2017
Author
Zwolak, A;Leettola, CN;Tam, SH;Goulet, DR;Derebe, MG;Pardinas, JR;Zheng, S;Decker, R;Emmell, E;Chiu, ML;
Journal
Sci Rep

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