G-CSF Protein Overview: Sequence, Structure, Function and Protein Interaction

G-CSF Protein Overview

Granulocyte colony-stimulating factor (or colony stimulating factor-3) specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes (Metcalf, 1985). Nagata et al. (1986) determined the partial amino acid sequence of purified GCSF protein, and by using oligonucleotides as probes, isolated several GCSF cDNA clones from a human squamous carcinoma cell line cDNA library. Cloning of human GCSF cDNA shows that a single gene codes for a 177- or 180-amino acid mature protein of molecular weight 19,600 (Nagata et al., 1986; Souza et al., 1986).

G-CSF protein family

Belongs to the IL-6 superfamily.

G-CSF protein name

Recommended name
Granulocyte colony-stimulating factor
Short name
G-CSF
Aliases
filgrastim, granulocyte colony stimulating factor, lenograstim, MGC45931, pluripoietin
Alternative name
Pluripoietin Filgrastim Lenograstim

G-CSF Protein Sequence

Species Human G-CSF protein
Length 207
Mass (Da) 22293
Sequence Human G-CSF protein sequence
Species Mouse G-CSF protein
Length 208
Mass (Da) 22421
Sequence Mouse G-CSF protein sequence
Species
Length
Mass (Da)
Sequence

G-CSF Protein Molecular Weight & PI

Granulocyte colony-stimulating factor precursor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 30-207 Granulocyte colony-stimulating factor.

Molecular weight (Da)

19058.06

Theoretical pI

5.43

G-CSF Protein Structure

2:2 COMPLEX OF G-CSF WITH ITS RECEPTOR
Deposited
1999-03-08   Released:  2000-03-08
Deposition Author(s)
Aritomi, M., Kunishima, N., Okamoto, T., Kuroki, R., Ota, Y., Morikawa, K.
Organism(s)
Mus musculus, Homo sapiens
Expression System
Spodoptera frugiperda, Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.5000 Å
R-Value Work
0.317
1PGR From PDB

Human G-CSF protein Secondary structure

G-CSF Protein Interaction

Recombinant G-CSF Protein Feature

G-CSF Protein, Human, Recombinant (Isoform b)

High Purity
> 95 % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
High Activity
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 50-200 pg/mL.

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