Iba1 Antibody Guideline: Preparation, Dilution and Applications

Iba1 Antibody Overview

There are 7 Iba1 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, ICC/IF, WB, IP. There are 6 Iba1 antibody for ELISA, 3 Iba1 antibody for IHC-P, 2 Iba1 antibody for ICC/IF, 2 Iba1 antibody for WB, 2 Iba1 antibody for IP. Among all these Iba1 antibodies, there are 1 anti-Iba1 mouse monoclonal antibodies , 2 anti-Iba1 rabbit monoclonal antibodies , 4 anti-Iba1 rabbit polyclonal antibodies . All the Iba1 anbodies are produced in house and all are in stock. Iba1 antibody customerized service is available.

Iba1 Antibody ELISA

Iba1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; Catalog#14227-H07E; P55008-1; Met1-Pro147). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.

Iba1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Iba1 Antibody western blot

Iba1 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; Catalog#14227-H07E; P55008-1; Met1-Pro147).

Iba1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Iba1 Antibody immunohistochemistry

Iba1 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; Catalog#14227-H07E; P55008-1; Met1-Pro147).

Iba1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

Iba1 Antibody immunoprecipitation

Iba1 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; Catalog#14227-H07E; P55008-1; Met1-Pro147). IBA1 / AIF1 specific IgG was purified by Human IBA1 / AIF1 affinity chromatography.

Iba1 Antibody immunoprecipitation Validated

Application IP
Dilution 0.1-0.5 μL/mg of lysate
Notes