MMP13 Protein Overview: Sequence, Structure, Function and Protein Interaction

MMP13 Protein Overview

Freije et al. (1994) cloned a cDNA coding for a novel human matrix metalloproteinase (MMP) from a cDNA library derived from a breast tumor. The isolated cDNA contains an open reading frame coding for a polypeptide of 471 amino acids. The predicted protein sequence displays extensive similarity to previously known MMPs and presented all the structural features characteristic of this protein family, including the well-conserved PRCGXPD motif. In addition, it contains in its amino acid sequence several residues specific to the collagenase subfamily (tyr214, asp235, and gly237) and lacks the 9-residue insertion present in the stromelysins. Because of the structural characteristics, Freije et al. (1994) called the new MMP collagenase-3 (CLG3), since it represented the third member of this family, composed of fibroblast (MMP1; 120353) and neutrophil (MMP8; 120355) collagenases. Northern blot analysis of RNA from normal and pathologic tissues demonstrated the existence in breast tumors of 3 different mRNA species, which seemed to be the result of utilization of different polyadenylation sites present in the 3-prime noncoding region of the gene. By contrast, no CLG3 mRNA was detected either by Northern blot or RNA polymerase chain reaction analysis with RNA from other human tissues, including normal breast, mammary fibroadenomas, liver, placenta, ovary, uterus, prostate, and parotid gland. A possible role for this metalloproteinase in the tumoral process was proposed.

MMP13 protein family

Belongs to the peptidase M10A family.

MMP13 protein name

Recommended name
Collagenase 3
Short name
MMP-13
Aliases
CLG3, collagenase 3
Alternative name
Matrix metalloproteinase-13

MMP13 Protein Sequence

Species Human MMP13 protein
Length 471
Mass (Da) 53820
Sequence Human MMP13 protein sequence
Species Mouse MMP13 protein
Length 472
Mass (Da) 54182
Sequence Mouse MMP13 protein sequence
Species Rat MMP13 protein
Length 466
Mass (Da) 53375
Sequence Rat MMP13 protein sequence

MMP13 Protein Molecular Weight & PI

Collagenase 3 precursor (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 104-471 Collagenase 3.

Molecular weight (Da)

42289.77

Theoretical pI

5.57

MMP13 Protein Structure

MMP13 Catalytic Domain Complexed with 4-{[1-methyl-2,4-dioxo-6-(3-phenylprop-1-yn-1-yl)-1,4-dihydroquinazolin-3(2H)-yl]methyl}benzoic acid
Deposited
2007-02-27   Released:  2007-07-24
Deposition Author(s)
Johnson, A.R., Pavlovsky, A.G., Ortwine, D.F., Prior, F., Man, C.-F., Bornemeier, D.A., Banotai, C.A., Mueller, W.T., McConnell, P., Yan, C.H., Baragi, V., Lesch, C., Roark, W.H., Lie, J.J., Fasquelle, V., Wilson, M., Robertson, D., Datta, K., Guzman, R., Han, H.-K., Dyer, R.D.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3000 Å
R-Value Free
0.340
R-Value Work
0.248
2OZR From PDB

Human MMP13 protein Secondary structure

MMP13 Protein Interaction

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